hydrophila has been carried out by amplification of aerolysin
gene, Hemolysin gene and lipase gene (5).
Van Dorsselaer, "Characterisation of the heptameric pore-forming complex of the Aeromonas toxin aerolysin
using MALDI-TOF mass spectrometry," FEBS Letters, vol.
hydrophila and is associated with aerolysin
; (7) it creates pores in the cell membrane, resulting in the cell death.
Construction of vaccine from Lactococcus lactis bacteria using Aeromonas hydrophila virulent Aerolysin
is the founding member of a superfamily of [beta]-pore-forming toxins whose pore structure is unknown.
Virulence of Aeromonads is considered to be multifactorial including cytotonic heat-labile (alt), and cytotonic heat-stable enterotoxins (ast), cytotoxic heat-labile enterotoxin (act), hemolysin (hly), aerolysin
(aei), flagella A and flagella B (fla), lipase (lip), elastase (ela), serine protease (ser), and DNases (exu) .
Aeromonas species can produce many virulence factors, including hemolysin, cytotoxin, aerolysin
, enterotoxin, endotoxin, protease, adhesins, leukocidin, and lipases.
hydrophila produces several extracellular products such as proteases, haemolysins, aerolysin
, cytolytic enterotoxins that are related with its pathogenicity (virulence) (Kingombe et al.,1999; 2010; Hu et al., 2012).Secreted extracellular hemolysin and cytolytic enterotoxin by bacteria are reported to be important for causing certain lytic activities in host cells (Watanabe et al.,2004; Uma et al., 2010).
The identity of Aeromonas species was determined by screening them for the presence of specific virulent genes aerolysin
(aerA) 34] and haemolysin (hylH) 35].
Other reasons for the elevation in the phagocytic index and ROS production recorded in the present study may be due to aerolysin
or cytotoxic enterotoxin (Act) secretions from A.
Lee, "Transport of a-helical peptides through a-hemolysin and aerolysin
pores," Biochemistry, vol.