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Standard solutions containing 100 pmol/L OHCbl, CNCbl, AdoCbl, and MeCbl (all from Sigma-Aldrich) were separated with HPLC to determine the retention times of the different Cbls.
Previous studies suggested that TC carries primarily OHCbl and to some degree AdoCbl (18).
4] Nonstandard abbreviations: Cbl, cobalamin; TC, transcobalamin; CNCbl, cyanocobalamin; OHCbl, hydroxocobalamin; AdoCbl, 5'-deoxyadenosylcobalamin; MeCbl, methylcobalamin; TC-CNCbl, TC-bound CNCbl.