It does not require cDNA cloning and blotting and can even be applied in laboratories where radioactive labelling is not available by using the silver staining method that enables the detection and recovery of the PCR products from acrylamide gels
All PCR products were run on a 10% acrylamide gel
to confirm the presence of insertions in both homozygous and heterozygous samples.
In addition, there are no hazardous reagents such as ethidium bromide, which is commonly used to visualize restriction digestion products on agarose or acrylamide gels
A reliable method for the recovery of DNA fragments from agarose and acrylamide gels
From these experiments it follows that PEG at 5 g/L is suitable for differential separation of single-stranded DNA fragments in the range of 150-528 nucleotides in 12% acrylamide gels with %C = 2.
By including PEG as an additive to acrylamide gels in SSCP analysis, it is possible to separate PCR amplicons in a broad size range, from 150 to 500 bp.
electrophoresis of soluble plant proteins.
Conventional methodology for detecting gene rearrangements consistent with T- and B-cell lymphomas involves electrophoresis of T-cell receptor-[gamma] (TCRG) and immunoglobulin heavy chain (IGH) gene rearrangement PCR products on an acrylamide gel
Most conformations seem to alter the physical configuration or size sufficiently that, even though the variant sequence has the same charge, the configuration-to-charge (size-to-charge) ratio is different enough to be detectable as a mobility difference upon electrophoresis through a retarding matrix such as acrylamide gel