polyacrylamide gel

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polyacrylamide gel

n.
A hydrated polymer consisting of a long chain of amide groups, used as a medium for substances that undergo gel electrophoresis.
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A total of 20 [micro]g of crude venom and 10 [micro]g of venom fractions were separated under reducing-conditions in a 10-20% Tricine acrylamide gel. The gel was run at 120V for 90 min using a BioRad PowerPak system.
Morulustatin, una desintegrina que inhibe la agregacion plaquetaria inducida por ADP, aislada de la serpiente de cascabel (Crotalus lepidus Morulus) de la roca de Tamaulipas, Mexico
NOTE: The main purpose of steps 4 and 5 is to observe the size and efficiency of PCR products amplified by different primer combinations and thus to estimate the electrophoresis time on 6% denaturing acrylamide gels in the next step.
SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) FOR STUDYING GENETIC DIVERSITY AND POPULATION STRUCTURE OF PLANTS AND OTHER LIVING ORGANISMS: A PROTOCOL
It does not require cDNA cloning and blotting and can even be applied in laboratories where radioactive labelling is not available by using the silver staining method that enables the detection and recovery of the PCR products from acrylamide gels.
Differential gene expression profiling in two cultivars of colza under aluminum stress (Brassica napus L.)
Concentrations of diffused solute from acrylamide gel were measured using disodium fluorescein and GGR as chemical tracers in the field.
Controlled Field Release of a Waterborne Chemical Signal Stimulates Planktonic Larvae to Settle
To confirm the results, PCR products were sequenced or separated on 10% acrylamide gels.
Melting temperature assay for a UGT1A gene variant in Gilbert syndrome
In addition, there are no hazardous reagents such as ethidium bromide, which is commonly used to visualize restriction digestion products on agarose or acrylamide gels. Moreover, because this method is sensitive and tolerates a broad range of DNA concentrations, there is a high rate for successful genotyping.
Allelic discrimination for single nucleotide polymorphisms in the human scavenger receptor class B type 1 gene locus using fluorescent probes
(1997) probably resulted from their use of acrylamide gels for allele detection.
Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system
The globulins and prolamines were resolved with 120 g [kg.sup.-1] and 100 g [kg.sup.-1] acrylamide gels, respectively.
Identification of tall fescue cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis of seed proteins
High percentage acrylamide gels improve resolution in SSCP analysis.
Single-strand conformation polymorphism analysis with high throughput modifications, and its use in mutation detection in familial hypercholesterolemia
From these experiments it follows that PEG at 5 g/L is suitable for differential separation of single-stranded DNA fragments in the range of 150-528 nucleotides in 12% acrylamide gels with %C = 2.5.
Optimization of single-strand conformation polymorphism analysis in the presence of polyethylene glycol
Acrylamide Gel electrophoresis of soluble plant proteins.
Biochemical characterization of soybean (Glycine max) TGX1485-1D seed proteins
The PCR products were electrophoresed in 3.5% agarose gel or 10.0% acrylamide gel (19:1 acrylamide/ bis-acrylamide) or 6.0% denaturing acrylamide gel (19:1 acrylamide/bis-acrylamide, 6 M urea).
QTL mapping of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean

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