A total of 20 [micro]g of crude venom and 10 [micro]g of venom fractions were separated under reducing-conditions in a 10-20% Tricine acrylamide gel
NOTE: The main purpose of steps 4 and 5 is to observe the size and efficiency of PCR products amplified by different primer combinations and thus to estimate the electrophoresis time on 6% denaturing acrylamide gels
in the next step.
It does not require cDNA cloning and blotting and can even be applied in laboratories where radioactive labelling is not available by using the silver staining method that enables the detection and recovery of the PCR products from acrylamide gels
Concentrations of diffused solute from acrylamide gel
were measured using disodium fluorescein and GGR as chemical tracers in the field.
The nondenaturing electrophoresis system has nearly the same resolution as that obtained with denaturing acrylamide gels
and silver staining.
To confirm the results, PCR products were sequenced or separated on 10% acrylamide gels
In addition, there are no hazardous reagents such as ethidium bromide, which is commonly used to visualize restriction digestion products on agarose or acrylamide gels
1997) probably resulted from their use of acrylamide gels
for allele detection.
A reliable method for the recovery of DNA fragments from agarose and acrylamide gels
High percentage acrylamide gels
improve resolution in SSCP analysis.
From these experiments it follows that PEG at 5 g/L is suitable for differential separation of single-stranded DNA fragments in the range of 150-528 nucleotides in 12% acrylamide gels
with %C = 2.