A total of 20 [micro]g of crude venom and 10 [micro]g of venom fractions were separated under reducing-conditions in a 10-20% Tricine acrylamide gel
. The gel was run at 120V for 90 min using a BioRad PowerPak system.
NOTE: The main purpose of steps 4 and 5 is to observe the size and efficiency of PCR products amplified by different primer combinations and thus to estimate the electrophoresis time on 6% denaturing acrylamide gels
in the next step.
It does not require cDNA cloning and blotting and can even be applied in laboratories where radioactive labelling is not available by using the silver staining method that enables the detection and recovery of the PCR products from acrylamide gels
Concentrations of diffused solute from acrylamide gel
were measured using disodium fluorescein and GGR as chemical tracers in the field.
To confirm the results, PCR products were sequenced or separated on 10% acrylamide gels
In addition, there are no hazardous reagents such as ethidium bromide, which is commonly used to visualize restriction digestion products on agarose or acrylamide gels
. Moreover, because this method is sensitive and tolerates a broad range of DNA concentrations, there is a high rate for successful genotyping.
(1997) probably resulted from their use of acrylamide gels
for allele detection.
The globulins and prolamines were resolved with 120 g [kg.sup.-1] and 100 g [kg.sup.-1] acrylamide gels
High percentage acrylamide gels
improve resolution in SSCP analysis.
From these experiments it follows that PEG at 5 g/L is suitable for differential separation of single-stranded DNA fragments in the range of 150-528 nucleotides in 12% acrylamide gels
with %C = 2.5.
electrophoresis of soluble plant proteins.
The PCR products were electrophoresed in 3.5% agarose gel or 10.0% acrylamide gel
(19:1 acrylamide/ bis-acrylamide) or 6.0% denaturing acrylamide gel
(19:1 acrylamide/bis-acrylamide, 6 M urea).