The buffers and the 20 enzyme systems analyzed were; 1) Clayton & Tretiak (1972) buffer: Aspartate amino transferase (AAT; 188.8.131.52), Aconitate hydratase
(ACO; 184.108.40.206), Malic enzyme (ME; 220.127.116.11), Glycerol-3phosphate dehydrogenase (G3PDH; 18.104.22.168), Isocitrate dehydrogenase (IDH; 22.214.171.124), Malate dehydrogenase (MDH; 126.96.36.199), Glucose-6-phosphate dehydrogenase (6PGD; 188.8.131.52), Phosphoglucomutase (PGM; 5.4.
The strains isolated were typed by multilocus enzyme electrophoresis using eight enzymatic systems: malate dehydrogenase (MDH, EC184.108.40.206), isocitrate dehydrogenase (IDH, EC220.127.116.11) with substrate NAD and NADP, malic enzyme (ME, EC18.104.22.168), glucose-6-phosphate dehydrogenase (G6PDH, EC22.214.171.124), 6-phosphogluconate dehydrogenase (6GPDH, EC126.96.36.199), Aconitate hydratase
(ACON, EC188.8.131.52), and hexokinase (HK, EC2.71.1) .
We analyzed seven different enzyme systems: aconitate hydratase
(Acn), diaphorase (Dia), isocitrate dehydrogenase (Idh), malate dehydrogenase (Mdh), phosphoglucoisomerase (Pgi), phosphoglucomutase, (Pgm) and Triose-phosphate isomerase (Tpi).
Basal metabolism Aconitate hydratase
Lipase Fructose-1,6-biphosphatase F-II Fructose-bisphosphate aldolase Alpha-amylase B 3-oxoacyl-ACP reductase C-4 sterol methyl oxidase 2 Amine oxidase Scavenger receptor cysteine-rich protein Vacuolar H+-ATPase C chain 4.