Pan et al., "His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase
," Biochemical and Biophysical Research Communications, vol.
Palmer et al., "Citrate enhances in vitro metastatic behaviours of PC-3M human prostate cancer cells: status of endogenous citrate and dependence on aconitase
and fatty acid synthase," International Journal of Biochemistry and Cell Biology, vol.
These findings are confirmed in an in vitro study showing that even if the aconitase
activity in basal condition is higher in fibroblasts than in cardiomyocytes, fibroblasts also have the highest aconitase
inactivation after DOX, resulting in cell mortality .
The levels of mitochondrial complexes and aconitase
were significantly increased in the model group (P < 0.05), while antioxidases were significantly decreased (P < 0.05), as compared to that in the control group.
Manganese inhibits mitochondrial aconitase
: a mechanism of manganese neurotoxicity.
MCA's mode of action is not well characterized, but it may exert its toxicity by attenuating the mitochondrial citric acid cycle via the formation of halocitrate and the subsequent inhibition of aconitase
(Frank et al., 1994).
(12) Similarly, elevated superoxide levels can inhibit the enzyme aconitase
, the rate-limiting step in generation of ATP via the Krebs cycle.
The activities of aconitase
were also reduced by 2 fold in response to frataxin deficiency.
The isomerization of citrate to isocitrate catalyzed by aconitase
is a key step in acid metabolism.
In case of low level of SOD2, the superoxide inactivates the aconitase
enzyme of the TCA cycle, ceases the energy metabolism, and releases the potentially toxic iron from aconitase
(Gardner et al., 1995).
Our results reveal that chronic mtDNA depletion in DRG neurons led to enhanced production of reactive oxygen species and decreases in the expression of aconitase
, which catalyzes the conversion of citrate to isocitrate.