Staining techniques for detection of acid fast bacilli
: what hope does fluorescein-diacetate (FDA) vitality staining technique represent for the monitoring of tuberculosis treatment in resource limited settings.
Microscopic examination showed 04 acid fast bacilli
/ 100 fields, histopathology revealed chronic granulomatous inflammation with necrosis and there was no evidence of malignancy.
The objective of this study was to detect and confirm the presence of acid fast bacilli
in lymph node biopsies using routine Ziehl Neelsen stain, Kinyoun stain and fluorescence staining and also to determine the best, rapid and more cost effective technique of staining for the detection of acid fast bacilli
Key words: Tuberculosis (TB), Mycobacterium tuberculosis (MTB), pulmonary tuberculosis (PTB), acid fast bacilli
(AFB), Ziehl Neelsen staining (ZN), Lowenstein-Jensen medium (LJ).
All tissue sections were stained with ZN stain (Ziehl-Neelsen) to visualize acid fast bacilli
He was treated for probable tuberculosis for 9 months, although the sputum studies were negative for acid fast bacilli
. There was no significant family history.
Mycobacterium tuberculosis (MTB) complex, an acid fast bacilli
(AFB), is the causative agent of TB.
However, even if the correct sample is expectorated, the bacillary population has to be at least 10000 per millilitre, to get the smear positive for acid fast bacilli
Key words: Acid fast bacilli
, smear, tuberculosis; pulmonary TB, sputum.
Furthermore, it has been demonstrated that acid fast bacilli
are excreted in the milk of not only lepromatous patients but also of tuberculoid, borderline-tuberculoid and borderline leprosy; and have also been demonstrated in non-lactating breast secretions.
The laboratory people must ensure examining at least 200-300 oil immersion fields in search of the acid fast bacilli
before reporting a sample negative for it.
Data was reviewed, including clinical history, availability of acid fast bacilli
stain on tissue and mycobacterium tuberculosis culture results.