Cultures came back positive for heavy growth of a nonfermenter later identified as Achromobacter xylosoxidans
, subspecies denitrificans.
A myringotomy was performed to release the right middle ear fluid collection which cultured positive for Achromobacter xylosoxidans
that was resistant to gentamicin.
However, in 3 samples, CF pathogens were identified only by NGS16S (Tables 1 and 2): Streptococcus agalactiae (specimen CF2, 9.55% of corrected reads), Achromobacter xylosoxidans
(specimen CF4, 10.26% of corrected reads), and Burkholderia cepacia complex (specimen CF15, 4.48% of corrected reads).
The microorganisms Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas aurantiaca , Salmonella typhi, Stenotroph maltophilia, Enterobacter cloacae, Enterobacter aerogenes, Klebsiella pneumoniae, Staphylococcus epidermidis, Bacillus subtilis and Bacillus megaterium were obtained from Children Hospital, Lahore, while Achromobacter xylosoxidans
, Azospirillum lipoferum and Citrobacter freundii, were obtained from biotechnology laboratory, FC College, Lahore.
is the most common bacillus in clinical samples and is recognized as an emerging and multidrug-resistant microorganism that causes various opportunistic infections and nosocomial outbreaks (3,6).
and Pseudomonas aeruginosa were identified using Vitek-2 GN (gram-negative) identification cards (Vitek 2 System, Biomerieux, USA).
Inoculation of plant growth promoting bacterium Achromobacter xylosoxidans
strain Ax10 for the improvement of copper phytoextraction by Brassica juncea.
2 (4) 0 Haemophillus influenzae 1 (2) 0 Stenotrophomonas maltophilia 1 (2) 1 (2) Klebsiella pneumoniae 0 1 (2) Tatumella ptyseos 0 1 (2) Achromobacter xylosoxidans
0 1 (2) Enterobacter cloacae 0 1 (2) Sphingobacterium multivorum 0 1 (2) Candida sp.
DNA from Pseudomonas stutzeri (ATCC 11607) served as a positive control for the amplification of the nirS amplicon while DNA from Achromobacter xylosoxidans
(ATCC 15173) served as a positive control for the amplification of the nirK amplicon.
PGPR2 strain encoded a LysR family transcriptional regulator (909 bp; Genbank accession number ASQO01000026.1, regions 2019 : 1111), which showed maximum homology with a LysR family regulator from Achromobacter xylosoxidans
(68% identity) in BlastP analysis.
MG1 strain has also been reported for malachite green decolorization.
Strain AYS3 showed 100% similarity with Achromobacter xylosoxidans
and found to be closely related to Achromobater denitrificans by phylogenetic analysis.