coli 3 0,9% 2 0,9% 0 0,0% Corynebacterium bovis 0 0,0% 0 0,0% 2 0,3% Acholeplasma
10 2,8% 0 0,0% 9 1,4% M.
Mouzykantov et al., "Extracellular vesicles derived from Acholeplasma
laidlawii PG8" TheScientificWorldJournal, vol.
AB084065 99 Cs1 Acholeplasma
axanthum U04657 90 Cs2 Polaribacter sp.
pirum and Acholeplasma
laidlawii, and the negative control samples were mycoplasma broth, Hut78 cell extract, and sterile RNase-free water.
The sequences of subgroup representatives obtained in this study as well as 30 phytoplasma strains available from GenBank, and Acholeplasma
palmae J233 (as outgroup) were aligned using ClustalW (Thompson, Higgins, & Gibson, 1994) in MEGA v.
van Deenen, "Polyene antibiotic-sterol interactions in membranes of Acholeplasma
laidlawii cells and lecithin liposomes.
Leach, "Further studies on classification of bovine strains of mycoplasmatales, with proposals for new species, Acholeplasma
modicum and Mycoplasma alkalescens," Journal of General Microbiology, vol.
Digitonin sensitivity test was carried out to differentiate between Mycoplasma and Acholeplasma
genera using filter paper discs impregnated with 0.2 mL of 1.5% (W/V) ethanol solution of digitonin and dried overnight.
The new 0.1 [micro]m version has been shown to fully retain Acholeplasma
laidlawii, whereas the 0.2 [micro]m completely retains Brevundimonas diminuta.
Our test protocol was based on the accepted HIMA methodology for testing sterilizing-grade filters[ii], modified by substituting Acholeplasma
laidlawii (ATCC no.
The 0.1 [micro]m Clariflow[R] filter alleviates concern about bacterial penetration of 0.2 [micro]m sterilizing-grade filters by demonstrating LRVs in excess of 11 after testing in accordance with the HIMA challenge methodology using Acholeplasma
laidlawii as the challenge organism.
Mycoplasmas and the related acholeplasmas
are one of the smallest (0.15 to 0.3 [micro]M) and self-replicating bacteria and are frequent contaminants of animal origin products.