Acanthamoeba polyphaga mimivirus (APM), named for its ameba host and bacteria-mimicking characteristics, is a double-stranded DNA virus with the largest viral genome described to date (1.
Acanthamoeba polyphaga mimivirus real-time PCR assays, was presented at the 23rd Annual Clinical Virology Symposium, Apr 29-May 2, 2007, in Clearwater Beach, Florida, USA.
Primer and probe sequences for Acanthamoeba polyphaga mimivirus real-time PCR assays Target Primer/probe Gene region APM-396 Forward Reverse Helicase Probe APM-596 Forward Reverse Thiol oxidoreductase Probe Target Sequence (5' [right arrow] '3') APM-396 ACC TGA TCC ACA TCC CAT AAC TAA A GGC CTC ATC AAC AAA TGG TTT C ACT CCA CCA CCT CCT TCT TCC ATA CCT TT APM-596 AAC AAT CGT CAT GGG AAT ATA GAA AT CTT TCC AGT ATC CCT GTT CTT CAA TTC GTC ATA TGC GAG AAA ATG CTA TCC CT Table 2.
Mesorhizobium amorphae, Rasbo bacterium, Azorhizobium caulinodans, Acanthamoeba polyphaga mimivirus, and conventional microorganisms in 210 pneumonia patients in intensive-care units by using culture, polymerase chain reaction, and serologic testing.
worsleiensis, Mesorhizobium amorphae, Rasbo bacterium, and Acanthamoeba polyphaga mimivirus (13,15).