Using DAVID, we found that hypermethylated genes in ESC cells are mostly enriched with the regulation of cell cycle (FZR1, E2F5, BOP1, TRRAP, CDK4, JUNB, etc.), cell death (SIVA1, MCL1, YPEL3, ARF6, UBQLN1, SHF, CIAPIN1, APLP1, GPX1, CASP3, etc.), and mRNA metabolic process (SCAF1, FIP1L1, STRAP, RBM15B, CWC15, XAB2, YBX1, AUH, SF3B2, APLP1, HNRNPL, etc.); the hypomethylated genes are enriched with functions related to ATP synthesis (ATP6V1F, ATP6V1C1, ATP6V0C
, ATP6V1A, ATP6V0E, ATP6V1E1, ATP5C1, etc.) and mitochondrial ribosome (MRPL15, MRPL27, MRPL16, MRPL36, MRPL39, MRPL34, DAP3, etc.) (Excel Sheet S2).
Pathway P value FDR Synaptic vesicle 3.36E - 08 3.58E - 05 cycle Rheumatoid 3.43E - 07 3.66E - 04 arthritis Collecting duct 2.29E - 08 2.44E - 05 acid secretion Pathway Nodes Synaptic vesicle ATP6V0C
, ATP6V1A, ATP6V0E1, ATP6V1E1, ATP6V1H, cycle ATP6V0A1, ATP6V1D Rheumatoid ATP6V0C
, ATP6V1A, ATP6V0E1, ATP6V1E1, ATP6V1H, arthritis ATP6V0A1, ATP6V1D Collecting duct ATP6V0C
, ATP6V1A, ATP6V0E1, ATP6V1E1, ATP6V0A1, acid secretion ATP6V1D Table 4: Cox multivariate analyses of biomarkers associated with OS in the generation and validation datasets.