We had established conditional CF-specific ATF3
overexpression mice (ATF3TGfl/flCol1a2-Cre+) and control mice (ATF3TGfl/flCol1a2-Cre−) as described previously, and all mice were housed in a specific pathogen-free level animal house in the Beijing Institute of Heart, Lung and Blood Vessel Disease.
Cyclic amp-dependent This protein binds the transcription factor ATF- camp response element 3 (CRE), present in many viral and cellular promoters, repressor of inflammatory response 7.
The antibodies against Chop, Atf3
, TATA box binding protein (Tbp), and HDAC1 were obtained from Santa Cruz Biotechnology (CA, USA).
33'-diindolylmethane induces activating transcription factor 3 (ATF3
) via ATF4 in human colorectal cancer cells.
It looks like the spread of breast cancer is associated with turning on a stress gene called ATF3
(Activating Transcription Factor 3).
Song et al., "A critical role of STAT1 in streptozotocin-induced diabetic liver injury in mice: controlled by ATF3
," Cellular Signalling, vol.
Amundson et al., "ATF3
induction following DNA damage is regulated by distinct signaling pathways and over-expression of ATF3
protein suppresses cells growth," Oncogene, vol.
The transcriptional regulator, ATF3
, plays a key role in this process.
Recent evidence claims that this factor can induce apoptosis in prostatic cancer cells via up-regulation of activating transcription factor 3 (ATF3
is a pro-apoptotic protein and a key mediator for apoptosis under stressful conditions.
Upregulation of the translation of ATF4 and other target proteins can then lead to increased transcription of stress-related genes (such as Atf3
, Asns, Cebpb, and Trib3), allowing the cell to synthesize the subset of proteins needed to respond to the stress that initiated the response [10-12].
Three genes were chosen for investigation based on their previously identified stress roles: Nuclear Factor Kappa p (NF[kappa]B), Tumor Necrosis Factor a (TNF[alpha]), and Activating transcription factor 3 (Atf3