A gene on chromosome 2q32 that encodes a member of the activation transcription factor/cAMP-responsive element-binding family. The gene is induced by various signals, including those evoked by cancer cells.
We had established conditional CF-specific ATF3 overexpression mice (ATF3TGfl/flCol1a2-Cre+) and control mice (ATF3TGfl/flCol1a2-Cre−) as described previously, and all mice were housed in a specific pathogen-free level animal house in the Beijing Institute of Heart, Lung and Blood Vessel Disease.
Recent evidence claims that this factor can induce apoptosis in prostatic cancer cells via up-regulation of activating transcription factor 3 (ATF3).1 ATF3 is a pro-apoptotic protein and a key mediator for apoptosis under stressful conditions.
Upregulation of the translation of ATF4 and other target proteins can then lead to increased transcription of stress-related genes (such as Atf3, Asns, Cebpb, and Trib3), allowing the cell to synthesize the subset of proteins needed to respond to the stress that initiated the response [10-12].
Three genes were chosen for investigation based on their previously identified stress roles: Nuclear Factor Kappa p (NF[kappa]B), Tumor Necrosis Factor a (TNF[alpha]), and Activating transcription factor 3 (Atf3).