Allele-specific PCR (AS-PCR
)  is commonly used to discriminate between alleles that have single-nucleotide variants (SNVs) and well-defined insertions and deletions (1, 2).
Genotyping: Allele-specific PCR (AS-PCR
) approach was followed as described by (Ye et al.
Genotyping was performed using the PCR-RLFP method for all SNPs except for rs1884444 in the IL23R gene and rs9645406 in ROR[gamma]t which were genotyped using the AS-PCR
method (see Table 2).
The TNF-[alpha] -308G/A polymorphism was genotyped by allele-specific polymerase chain reaction (AS-PCR
However, the existing experimental methods for haplotyping, such as single-molecule dilution , cloning , and allele-specific polymerase chain reaction (AS-PCR
) , are more laborious, complex, and expensive than genotyping [7 ].
In this descriptive study, out of 98 clinical isolates of Mycobacterium tuberculosis at The Tuberculosis Research Center in the Iranian city of Arak, 64 isolates that had already been determined to be phenotypically resistant or susceptible to isoniazid were analyzed for mutations in the regulatory region of inhA-15 with 2 allele-specific polymerase chain reaction (AS-PCR
) and sequence methods.
The remaining 2 SNPs could not be typed using restriction enzymes and were, thus, genotyped by Allele Specific PCR (AS-PCR
Sanger sequencing shows a generally low sensitivity, with restriction-enzyme digestion and allele-specific PCR (AS-PCR
) strategies typically achieving higher detection rates.
Allele-specific PCR assay (AS-PCR
) has been developed as a tool to detect single nucleotide changes that result in target site insensitivity in the resistant population.
Jouve, "Molecular characterisation of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR
markers for HMW glutenins of wheat," Theoretical and Applied Genetics, vol.
Hence, we investigated the nucleotide polymorphism in the albendazole-binding domain of the isotype 1 [beta]-tubulin gene from several populations of Wuchereria bancrofti and developed an AS-PCR
assay useful in screening for sensitive/resistance alleles among parasite populations and also evaluated its utility.
Several recently developed methodologies, such as allele-specific amplification refractory mutation system PCR (ARMS), bead emulsification amplification and magnetics (BEAMing) technology, allele-specific PCR (AS-PCR
), droplet digital PCR (ddPCR), and next generation sequencing (NGS), have been used to detect and quantify rare variants in the blood of melanoma patients, with analytical sensitivity ranging from 0.005 to 5% (Table 1).