Overexpression of histone phosphorylation proteins such as ARK1
and ARK2 and histone-modifying genes, such as histone methyltransferases G9a, EZH2, and SUV39HZ, in HCC tissues predicts tissue invasion and poor prognosis [130, 156].
Figure 1 shows the docked enzyme-substrate complex for ArK1. Of these 26 substrates only two had their stereochemistry incorrectly predicted (ArK18 and ArK22) by the model.
This enzyme displays the anti-Prelog rule 21 out of 26 times for the ArKs (Table 1), providing an explanation for why the stereochemistry is seen to reverse in the series ArK1 to ArK6.
The Aurora-A is encoded by the AURKA gene (also known as AIK, Aurora/IPL1-like kinase; ARK1
, Aurora related kinase 1; AURA, AURORA2; BTAK, breast tumoramplified kinase; PPP1R47, protein phosphatase 1 regulatory subunit 47; STK15, serine/threonine-protein kinase 15; STK6, serine/threonine kinase 6), located at 20q13.2 and consisting of 11 exons (Gene ID: 6790).
The researchers targeted the beta-adrenergic kinase (beta Ark1
) enzyme that is elevated in human heart failure.
Seventeen enzyme loci were scored: Aco-2 (encoding aconitase), Ark1
, Ark-2 (arginine kinase), Dia-2 (NADH diaphorase), Eno (enolase), Gapd (glycerol-3-phosphate dehydrogenase), Got1, Got-2 (glutamate-oxaloacetate transaminase), Gpi (glucosephosphate isomerase), Gpt (glutamate-pyruvate transaminase), Idh-1, Idh-2 (isocitrate dehydrogenase), Mdh-2 (malate dehydrogenase), Mpi (mannosephosphate isomerase), Pep-2 (dipeptidase), Pgm (phosphoglucomutase) and Tpi (triosephosphate isomerase).