Alleles of the ApoE gene
have been implicated in susceptibility to cardiovascular disease (17, 18) and sporadic late-onset Alzheimer disease (19-21); some reports have indicated that expression of the ApoE alleles may be differentially regulated (22, 23).
APOE genotyping was performed with the primer set F6/F4 (Amersham/Pharmacia Biotech Inc.), which amplifies part of exon 4 of the APOE gene
, including the coding sequence for amino acid residues 112 and 158 (11).
ApoE is encoded by a polymorphic gene on chromosome 19 (the APOE gene
), and three alleles ([member of]2, [member of]3, and [member of]4) have been described.
amplification and typing were performed as described by Saunders et al.
The sequences of oligonucleotides oligo 3 and oligo 4 were identical to the one around codon 112 of the APOE gene
; oligonucleotides oligo 3b and oligo 2 are the same as the sequence around codon 158 of the APOE gene
Hegele's finding of unique disease-causing mutations in the APOE gene
during routine genotyping for Alzheimer disease highlights the continuing need for broad patient-oriented clinical observation and intervention as genetic discoveries and tests move from the research laboratory into clinical use.
Our data suggest that women who inherit distinct alleles of the APOE gene
may require individualized ERT or combination therapy.
In familial defective apolipoprotein B-100, the clearance of LDL particles from the circulation is impaired because of reduced affinity of the apolipoprotein (apo) B component of LDL for the LDL receptor as a result of a G-to-A mutation at nucleotide 10708 in exon 26 of the apoE gene
, which causes substitution of [Arg.sup.3500] for Gln (1, 2).
In one, they focused on the APOE gene
; certain variants of that gene are linked to a relatively higher risk of Alzheimer's disease.
Genotyping, particularly of ApoE gene
alleles is also useful in the evaluation of cases and planning management.
To establish ApoE knock-out mice lines, Piedrahita with co-authors disrupted ApoE gene
in mouse ES cells using electroporation with plasmids of the replacement type to induce HR, after which, modified ES cells were injected into mouse blastocysts.
The APOE gene
encodes apolipoprotein E, a lipid-transporting protein in the brain.