The tetranucleotide GTTT was added to the 5' end of the reverse primers of DM45050, DM45209, DM45958, DM46513, DM47004.1, and BV209569, while the heptanucleotide GTTTCTT was added to the reverse primer of APOC2.
The final panel comprised 5 upstream (D19S112, DM46513, DM46892, DM47004.1, and BV209569) and 7 downstream markers (DM45050, DM45178, DM45209, D19S559, APOC2, D19S543, and DM45958).
Molecular analysis of apolipoprotein E (APOE), apolipoprotein C-11 (APOC2
), lipoprotein lipase (LPL), or lipase A (lysosomal acid, cholesterol esterase, LIPA) genes may be required, in addition to histopathological evaluation of the spleen.
In contrast, peptides from the following apolipoproteins were identified in the lipoprotein particle fractions: ApoA1, ApoA2, ApoA4, ApoB, ApoC1, ApoC2
, ApoC3, ApoC4, ApoD, ApoE, ApoF, ApoL, ApoM, and lipoprotein(a).