The administration of AMP deaminase
partly reversed the methotrexate-mediated reduction in leukocyte accumulation.
In this biosynthetic pathway, purine nucleoside phosphorylase (punA) that converts adenosine to adenine, adenosine kinase that converts adenosine to adenosine 5'-monophosphate (AMP), adenine phosphoribosyltransferase (APRT) that converts adenine to AMP, AMP deaminase that converts AMP to IMP, inosine monophosphate (IMP) dehydrogenase that converts IMP to XMP, guanosine monophosphate (GMP) synthase that converts XMP to GMP, and guanine deaminase that converts GMP to guanine were, respectively, determined (Figure 1).
One punA gene, one adenosine kinase gene, one APRT gene, two AMP deaminase genes, two IMP dehydrogenase genes, three GMP synthase genes, and two guanine deaminase genes were successfully cloned (Figure S3, Supporting Information), and the corresponding proteins expressed in E.
It was proposed that the activity of this enzyme would be inhibited after nitrogen depletion, as a result of significant decrease in AMP concentration via the activation of AMP deaminase; its activity is dependent on AMP concentration.
Diminishing activity of NAD: ICDH through repression or significant decrease in intracellular AMP concentration via the activation of AMP deaminase would result in the accumulation of citrate which serves as the precursor for ATP citrate lyase (ACL).
The net effect of this inhibition is the accumulation of AICAR and its metabolites, which are inhibitors of adenosine deaminase and AMP deaminase
. (1,2) By reducing the catabolism of adenosine and adenine nucleotides, adenosine levels increase both directly and indirectly from dephosphorylation of AMP, particularly by 5'-ectonucleotidase.
The enzyme AMP deaminase
(AMPD) (EC 188.8.131.52.) catalyzes the deamination of AMP to ammonia (N[H.sub.3]) and inosine monophosphate (IMP) in working skeletal muscle.
Diversos autores corroboram essa analise (Graham e Colaboradores, 1997; Wagenmakers, 1998, Bassini-Cameron e Colaboradores, 2008), quando afirmam que individuos treinados apresentam menor elevacao da amonemia quando comparados com nao treinados, ou treinados em atividades anaerobias (Yuan e Colaboradores, 2002), fato explicado possivelmente pela reducao da atividade das enzimas AMP deaminase
e glutamato desidrogenase.
IMP and AMP deaminase
in reperfusion injury down-regulates neutrophil recruitment.
AMP is usually deaminated in most tissues by AMP deaminase
and, to a lesser extent, by the ADA degradation pathway.
Dale's rabbit studies also showed that the amount of AMP deaminase
, an enzyme that degrades a component of ATP, declines in older red cells -- a finding since confirmed in human red cells.
oryzae (E value= 0) and with AMP deaminases
of both A.