For the present study, we chose well-known genes, regulation regions of which were often hypermethylated at colorectal cancer: SEPT9, FBN1, VIM, SDC2, THBD, SFRP2, ESR1, TMEFF2, NGFR, ALX4, HLTF, and NEUROG1 [20,21].
For example, we can observe such results in literature for SEPT9, ALX4, RASSF1, and some other genes [20,21].
These markers (ALX4
, EYA4, FOXL2, and SIX6) will be tested in subsequent studies to evaluate their clinical performance.
Also, other genes have been reported to be highly specific and sensitive cfDNA CRC biomarkers, such as APC, CDKN2A/P16h, MLH1, ALX4
, TMEFF2, NGFR, FRP2, NEUROG1, TPEF/HPP1, and RUNX3 [72-74].
The skull, known as Xujiayao 11, has an unusual perforation through the top of the brain case-an enlarged parietal foramen (EPF) or "hole in the skull"-that is consistent with modern humans diagnosed with a rare genetic mutation in the homeobox genes ALX4
on chromosome 11 and MSX2 on chromosome 5.