Membrane were incubated with primary antibodies anti-phospho-Akt (Ser473) (1:500 dilution, Cell Signaling Technology), anti- Akt
(1:1000 dilution), anti-pAkt (1:1000 dilution), and anti-tubulin (1:2000 dilution).
Formononetin inhibits migration and invasion of mda-mb-231 and 4t1 breast cancer cells by suppressing mmp-2 and mmp-9 through pi3k/ akt
It also activates Akt
, thereby enhancing mTORC1 functions.
An EGFR and AKT
Signaling Pathway was Identified with Mediation Model in Osteosarcomas Clinical Study.
has more recently been identified as a key player in promoting "synaptic plasticity," the brain's ability to strengthen cellular connections in response to experience.
Recent studies displayed that AKT
signaling and PPAR[gamma] have a mutual inhibitory effect [25-27], but this theory is still controversial.
binds PIP3 which enables 3-phosphoinositide-dependent kinase-1 (PDK1) to phosphorylate AKT
Changes of Akt
, p-Akt, GSK-3[beta], and p-GSK-3[beta] Protein Levels in the Myocardial Tissue.
observed that treatment of GBM cell lines with rapamycin not only resulted in a time-dependent decrease of S6K phosphorylation but also caused a paradoxical increase of AKT
phosphorylation on serine 473 which is known to be responsible for cell proliferation.
SC79 (Abcam, ab146428) was used as an AKT
A rabbit polyclonal antibody against rat Akt
(1 : 300, Abcam, USA), a rabbit monoclonal antibody against rat NF-[kappa]B p50 (1:100, Abcam, USA), a rabbit polyclonal antibody against rat Bax (1:50, Abcam, USA), and a rabbit polyclonal antibody against rat Bcl-2 (1 : 100, Cell Signaling Technology, USA) were used as primary antibodies.
Also termed RAC-PK ("related to the A and C kinases"), the Akt
isoforms comprise a family of three members -Akt1 (PKB-[alpha]), Akt2 (PKB-[beta]), and Akt3 (PKB[gamma])--and their activities are very susceptible to metabolic alterations such as obesity, insulin resistance, and type 2 diabetes (18).