Among those genes, Sp1, Tgfbr1 , and Rock1 belong to the TGF-[sz] signaling pathway; Bmpr1a belongs to the BMP signaling pathway; Fos is from the osteoclast differentiation pathway; Lrp6 is from the Wnt signaling pathway; and Adra1b
belongs to the calcium signaling pathway.
Previous studies have demonstrated adrenergic, alpha-1B-, receptor (ADRA1B) and peroxisome proliferator-activated receptor gamma, coactivator 1 beta (PPARGC1B) genes are involved in the regulation of ovarian follicular development, selection and maturation in broilers and rats (Aguado and Ojeda, 1986; Tcherepanova et al., 2000; McDerment et al., 2012), with a potential effect on egg production in laying hens.
Gene ADRA1B encodes alpha-1B adrenergic receptor ([alpha]1B ADR) that is a member of the G-protein-coupled family of transmembrane receptors (Allen et al., 1991), located at chromosome 13 in chickens (Gene ID: 373890).
Therefore, it was inferred that variations of PPARGC1B and ADRA1B gene sequences may greatly influence chicken ovarian development and egg laying performance, although the molecular mechanisms of both of them in regulating follicle development and growth remain unknown.
In this study, ADRA1B and PPARGC1B fragments were examined for novel sequence polymorphism using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) approach and sequencing analysis.
Primers were designed based the ADRA1B (GenBank accession No.
The PCR conditions included: 94[degrees]C for 2 min, followed by 35 cycles at 94[degrees]C for 30 s for denaturing, 59[degrees]C (55[degrees]C) for 30 s for annealing of ADRA1B gene (PPARGC1B gene), 72[degrees]C for 30 s for extension, and a final extension at 72[degrees]C for 2 min.
nih.gov/blast/bl2seq/bl2.html) to confirm the expected chicken ADRA1B and PPARGC1B gene sequences.
To screen for ADRA1B and PPARGC1B gene polymorphism, the confirmed PCR products were further analyzed using the SSCP assay as previously described (Qin et al., 2015b).
HTR2C, CHRM2, ADRA2B, HTR6, HTR5A, ADRA2C, HTR7, DRD2, and DRD4; SLC6A4, ADRA1A, HTR1A, ADRA1B
, HTR2A, and DRD1; OPRL1, CHRM5, CHRM1, ADRB1, ADRA2A, and HRH1; and CHRM4, CHRM3, KCNH2, SLC6A4, CYP2D6, SCN1A: four clusters of neuronal GPCRs (G-proteincoupled receptors: adrenergic, cholinergic, histamine, and serotonin receptors).
A high density linkage disequilibrium mapping in 14 noradrenergic genes: evidence of association between SLC6A2, ADRA1B
Category Group Regulated core genes Cardiac hypertrophy WB TPM1.FGF23 Cardiac hypertrophy Imipramine EPO, UCN2, TERT, VDR, ACE Cardiac arrhythmia lmipramine ATP1A3, SCN1A, KCNH7, KCNG2, KCNQ1, ADORA1, ADRA1B
, ADRB3, ADRA1D Cardiac adenopathy EtOH-Fr DLCAP4, MTHFS, EVC2, MAPK9.