Gene ADRA1B encodes alpha-1B adrenergic receptor ([alpha]1B ADR) that is a member of the G-protein-coupled family of transmembrane receptors (Allen et al.
Therefore, it was inferred that variations of PPARGC1B and ADRA1B gene sequences may greatly influence chicken ovarian development and egg laying performance, although the molecular mechanisms of both of them in regulating follicle development and growth remain unknown.
In this study, ADRA1B and PPARGC1B fragments were examined for novel sequence polymorphism using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) approach and sequencing analysis.
Primers were designed based the ADRA1B (GenBank accession No.
The PCR conditions included: 94[degrees]C for 2 min, followed by 35 cycles at 94[degrees]C for 30 s for denaturing, 59[degrees]C (55[degrees]C) for 30 s for annealing of ADRA1B gene (PPARGC1B gene), 72[degrees]C for 30 s for extension, and a final extension at 72[degrees]C for 2 min.
html) to confirm the expected chicken ADRA1B and PPARGC1B gene sequences.
To screen for ADRA1B and PPARGC1B gene polymorphism, the confirmed PCR products were further analyzed using the SSCP assay as previously described (Qin et al.
A 389 bp PCR amplicon obtained for ADRA1B gene and a 276 bp fragment for PPARGC1B were cloned using specifically designed primers, with the obtained fragments sequenced.
In the study, genotyping of the amplified ADRA1B and PPARGC1B target fragments was conducted by PCR-SSCP analysis.
HTR2C, CHRM2, ADRA2B, HTR6, HTR5A, ADRA2C, HTR7, DRD2, and DRD4; SLC6A4, ADRA1A, HTR1A, ADRA1B
, HTR2A, and DRD1; OPRL1, CHRM5, CHRM1, ADRB1, ADRA2A, and HRH1; and CHRM4, CHRM3, KCNH2, SLC6A4, CYP2D6, SCN1A: four clusters of neuronal GPCRs (G-proteincoupled receptors: adrenergic, cholinergic, histamine, and serotonin receptors).
A high density linkage disequilibrium mapping in 14 noradrenergic genes: evidence of association between SLC6A2, ADRA1B
FGF23 Cardiac hypertrophy Imipramine EPO, UCN2, TERT, VDR, ACE Cardiac arrhythmia lmipramine ATP1A3, SCN1A, KCNH7, KCNG2, KCNQ1, ADORA1, ADRA1B
, ADRB3, ADRA1D Cardiac adenopathy EtOH-Fr DLCAP4, MTHFS, EVC2, MAPK9.