Genetic variation in alcohol dehydrogenase (ADH1A, ADH1B, ADH1C, ADH7
) and aldehyde dehydrogenase (ALDH2), alcohol consumption and gastric cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort.
The specific amount of DNA was quantified by taking the spectrophotometer readings at a wavelength of 260 nm Primers used in SSR and ADH-specific primer Analysis are SCYOR267C, C5, C11, SC8132X, ADH1, ADH3, ADH4, ADH7
variation modulates extraversion and conscientiousness in substance-dependent subjects.
More recent evidence suggests that genetic variants in the ADHIA, ADHIB, ADHI C ADHS, ADH6, and ADH7
genes are associated with illicit drug dependence and that this association is not purely attributable to co-morbid alcohol dependence (Luo et al.
Finally, variations in the ADH7
gene may play a protective role against alcoholism through epistatic effects.
The subunits are encoded by six different genetic loci (ADH1A, ADH1B*1, ADH1C*1, and ADH4-ADH7--formerly ADH1 through ADH7
No Locus Primers 1 SCYOR267C TAC TAA CGT CAA CAC TGC TGC CAA GGA TCT ACT TGC AGT ATA CGG G 2 C5 TGA CAC AAT AGC AAT GGC CTT CA GCA AGC GAC TAG AAC AAC AAT CAC A 3 C11 TTC CAT CAT AAC CGT CGT GGA TT TGC CTT TTT CTT AGA TGG GCT TTC 4 SC8132X CTG CTC AAC TTG TGA TGG GTT TTG G CCT CGT TAC TAT CGT CTT CAT CTT GC 5 ADH1 ATG TCT ATT CCA GAA ACT CAA AA TTA TTT AGA AGT GTC AAC AAC GT 6 ADH3 ATG TTG AGA ACG TCA ACA TTG TTA TTT ACT AGT ATC GAC GAC G 7 ADH4 TTA ATA TTC ATA GGC TTT C ATG TCT TCC GTT ACT GGG TT 8 ADH7
CTA TTT ATG GAA TTT CTT ATC ATG GGG TAG CCA CTC GAC AAA
An association study by Osier and colleagues (2004) found a potential epistatic interaction between the ADHIB and ADH7
genes among a Han Chinese population.
Eight primers (Table-1) of yeast were successful in amplifying DNA in the sample viz., ADH1, ADH2, ADH3, ADH4, ADH6, ADH5, ADH7
and ADH8 primers, Saccharomyces sppstrains used on wine production identifying variations at DNA level among the yeasts (Raton, 2004).
For example, several studies found other variations in and near the ADH1B gene, as well as in or near the ADH4, ADH1C, ADH5, ADH6, and ADH7
genes that affect risk for alcoholism or the level of alcohol consumption (see the article by Hurley and Edenberg, pp.
Humans have seven different genes, called ADH1A, ADH1B, ADH1C, ADH4, ADH5, ADH6, and ADH7
, that encode medium-chain ADHs (see Table 1).
* The ADH7
gene has a limited expression pattern and mainly is found in endothelial cells, such as those lining the esophageal and stomach tissues, as well as during embryonic development when it may contribute to the metabolism of retinol, a form of vitamin A (Hurley et al.