ADAMTSaf5

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ADAMTSaf5

An ADAMTSs family enzyme encoded by ADAMTS5 on chromosome 21q21.3 which, like ADAMTS4, cleaves aggrecan, the major proteoglycan of cartilage. It is highly expressed in placenta and appears to play a role in proteolytic processing during the peri-implantation period. It may be upregulated in arthritic diseases.
References in periodicals archive ?
Genetic variation including nonsynonymous polymorphisms of a major aggrecanase, ADAMTS-5, in susceptibility to osteoarthritis.
The breakdown of AGC is believed to be initiated by a disintegrin and metalloproteinase with thrombospondin-like motifs (ADAMTS)-4 and ADAMTS-5.[1]
Brevican is also degraded by various MMPs and ADAMTS proteases [56, 57], including ADAMTS-4 [58] and ADAMTS-5 [59].
Small Molecules to Inhibit ADAMTS-5 for Osteoarthritis 31
Shi, "Expression of ADAMTs-5 and TIMP-3 in the condylar cartilage of rats induced by experimentally created osteoarthritis," Archives of Oral Biology, vol.
In the EGCG-treated (intraperitoneal injection) mice, articular cartilage shows downregulation of MMP-1, MMP-3, MMP-8, MMP-13, ADAMTS-5, IL-1[beta], and TNF[alpha] mRNA and upregulation of CBP/p300 interacting transactivator with ED-rich tail 2 (CITED2), which suppresses MMPs transcription [31].
The expression levels of MMP-1, MMP-3, MMP13, ADAMTS-4, ADAMTS-5, collagen type II alpha 1 chain (COL2A1), cartilage oligomeric matrix protein (COMP), collagen type I alpha 2 chain (COL1A2), and collagen type X alpha 1 chain (COL10A1) mRNA in the articular cartilage were determined by RT-PCR assays using a SYBR green kit (Sigma-Aldrich) according to the method described by Takahito [21] and Hyuck [22].
After 24 hrs of stimulation with DAMPs, culture media were dialyzed and concentrated for examination of MMP-1, MMP-3, MMP-13, ADAMTS-5, and ADAM-8.
The primary antibodies included rabbit polyclonal antibodies specific for ADAMTS-1 (1:50; Abcam, Cambridge, United Kingdom), ADAMTS-4 (1:50; AVIVA Systems Biology, San Diego, California), ADAMTS-5 (1:100; Novus, Littleton, Colorado), ADAMTS-14 (1:100, Abcam), and rabbit polyclonal IgG purified from preimmune serum samples (1:50 or 1:100, ab27478; Abcam).
During the OA process, aggrecans are cleaved at specific Glu-Xaa peptide bonds by aggrecanases such as ADAMTS-4 and ADAMTS-5, resulting in a breakdown of HA and link proteins and a decrease in stable ternary complexes in the extracellular matrix [9].
Results: We report that low mass 4.5-kDa HA-fs enhance MMPs, including MMP -3 and 13 but not an ADAMTS-5 in chondrocytes and cartilage explant cultures, to a lesser extent than a Fn-f, included as a positive control, but enhance cartilage matrix damage to a similar extent as Fn-f.
It is also suggested that another enzyme, aggrecanase-2, or ADAMTS-5 (a disintegrin and MMP domain with thrombospondin motifs), plays a predominant role in the proteolysis of OA cartilage aggrecan.