(2003) IL-1 beta induces COX2, MMP-1, -3 and -13, ADAMTS-4
, IL-1 beta and IL-6 in human tendon cells.
Expression of ADAMTS-4
by chondrocytes in the surface zone of human osteoarthritic cartilage is regulated by epigenetic DNA de-methylation.
To investigate the effect of mechanical loading on AGC, ADAMTS-4
, and ADAMTS-5 , mRNA expression in human chondrocytes was quantified using real-time polymerase chain reaction.
The expression levels of ADAMTS-4
, ADAMTS-8, ADAMTS-10, AD-AMTS-13, and ADAMTS-14 were found to be significantly lower in preeclamptic placentas than in control placentas (Figure 1).
Brevican is also degraded by various MMPs and ADAMTS proteases [56, 57], including ADAMTS-4
 and ADAMTS-5 .
When stimulated there is an increase in cell expression in the chondrocytes of several matrix metalloproteinases (MMPs) such as MMP 1, 3 and 13, and prostaglandin E-2 (PGE-2), and aggrecanases such as a disintegrin and metalloproteinase with thrombospondin motif 4 (ADAMTS-4
Consistent with our result, Richards et al (27) demonstrated that versican was the primary substrate in COCs not only for ADAMTS-1 but also for ADAMTS-4
and 5, and they were all expressed in spatiotemporal patterns suggesting evident and probable overlapping functions with each other.
Kimura et al., "CCN1 (Cyr61) is overexpressed in human osteoarthritic cartilage and inhibits ADAMTS-4
(aggrecanase 1) activity," Arthritis & Rhematology, vol.
and ADAMTS-5: key enzymes in osteoarthritis," Journal of Cellular Biochemistry, vol.
For instance, miR127-5p was shown to target osteopontin (OPN), an important regulator of OA-related factors, such as MMP-13, TIMPs, and ADAMTS-4
, in human chondrocytes .
Sanmiao formula, a Chinese traditional medicinal prescription since the Ming Dynasty, exhibits protective activity for cartilage through decreasing the expression of MMP-3 and ADAMTS-4
and augmenting the expression of TIMP-1 and TIMP-3.
The expression levels of MMP-1, MMP-3, MMP13, ADAMTS-4
, ADAMTS-5, collagen type II alpha 1 chain (COL2A1), cartilage oligomeric matrix protein (COMP), collagen type I alpha 2 chain (COL1A2), and collagen type X alpha 1 chain (COL10A1) mRNA in the articular cartilage were determined by RT-PCR assays using a SYBR green kit (Sigma-Aldrich) according to the method described by Takahito  and Hyuck .