The expression levels of ADAMTS-4, ADAMTS-8, ADAMTS-10, AD-AMTS-13, and ADAMTS-14
were found to be significantly lower in preeclamptic placentas than in control placentas (Figure 1).
The primary antibodies included rabbit polyclonal antibodies specific for ADAMTS-1 (1:50; Abcam, Cambridge, United Kingdom), ADAMTS-4 (1:50; AVIVA Systems Biology, San Diego, California), ADAMTS-5 (1:100; Novus, Littleton, Colorado), ADAMTS-14 (1:100, Abcam), and rabbit polyclonal IgG purified from preimmune serum samples (1:50 or 1:100, ab27478; Abcam).
ADAMTS-14 was expressed at intermediate levels in the cytoplasm of all 3 types of trophoblasts and was weakly but positively expressed by the villous stromal cells compared with that of a negative control (Figure 1, E and F).
In the second-trimester and third-trimester placentas, the patterns and intensities of ADAMTS-1 and ADAMTS-14 expression in the 5 cell types were similar to those in the first-trimester placenta.
ADAMTS-14 showed consistently intermediate positivity in all cases of gestational trophoblastic diseases (data not shown).
Among them, ADAMTS-1, -4, and -5 showed different expression patterns between normal placental tissue and gestational trophoblastic diseases, while the staining patterns of ADAMTS-14 in placentas obtained from patients diagnosed as having gestational trophoblastic diseases did not differ from the staining patterns observed in normal placenta.
Association of a nsSNP in ADAMTS-14 to some osteoarthritis phenotypes.
E, ADAMTS-14 immunohistochemical staining shows intermediate cytoplasmic positivity in syncytiotrophoblasts, cytotrophoblasts, and intermediate trophoblasts.