The PDILT gene, part of the PDI family, helps another gene product called ADAM3
to form and assemble correctly, and then to reach the surface of a sperm, thereby equipping it with the right tools and machinery to navigate and make contact with an egg.
For ADAM3 immunocytochemistry, sperm were attached to a glass slide by cyiospin centrifugation, and the attached sperm samples were fixed with methanol at RT for 5 min and immunostained with anti-ADAM3 antibody.
Furthermore, protein analysis of ADAM3, a sperm surface protein associated with the fertilization process (Nishimura et al.
Our results indicate that B[^]P can disrupt testosterone production in Leydig cells, which may ultimately lead to a decrease in sperm quality in the epididymis via reduced levels of sperm motility, acrosomal integrity, and fertility-related proteins such as ADAM3 (Nishimura et al.