ACTB


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ACTB

A gene on chromosome 7p22 that encodes beta actin, which coexists with gamma actins as a major cytoskeletal component and mediator of internal cell motility. ACTB is also an older gene name for what is formally (per HUGO) known as ACTG1, see there.
References in periodicals archive ?
Equal amounts of solubilized cellular proteins were separated by SDSPAGE and immunoblotted with antibodies against FABP4 and ACTB as a loading control.
The reference genes B2M, ACTB, and EF-1a, which have been commonly used in numerous homologous species studies, were chosen in the present study and specific primers were designed in our laboratory to obtain a qPCR product of approximately 120-300 bp in length and avoid hairpin and cross dimer.
b) POTEE protein in complex with TUBA1A is the heavier protein complex that coimmunoprecipitates with both RPeL27 and RPeL43, respectively, while the lighter complex was ACTB and ACTBL2 protein.
The amendment to section 355's ACTB rule was generally effective as of the date of the enactment of the legislation.
The primers generated amplicons with the following sizes: EPCAM, 395 bp; MUC1, 293 bp; MUC16,432 bp; ERCC1, 366 bp; and ACTB, 114 bp.
Genespecific primers for B2M, ACTB, GAPDH, RPL27, HPRT1 and OAZ1, as well as a PCR positive control and a genomic DNA control were fixed in 96-well PCR plate.
For the reference genes, the forward and reverse primers were respectively 5-ACTCTTCCAGCCTTC CTTCC-3' and 5'-GGCAGGACTTAGCTT CCACA-3' for ACTB, and 5'-GGAAT GTGGGCTTTGTGTTC-3' and 5'-CCCAA TTGTCCCCTTACCTT-3 ' for RPLP0.
5 was employed to design a set of primer pair and specific TaqMan probe for amplification and detection of a 414 bp fragment of human ACTB (actin, beta) as an endogenous fragment.
Samples were analyzed in triplicate, and SIRT3 expression levels were normalized to the corresponding ACTB ([beta]-actin) levels.
Of five selected reference genes (Figure 3), abundance of ACTB and HPRT was slightly higher at 1.
The data were normalized across all plates to the following housekeeping genes: ACTB, B2M, GAPDH, HPRT1, HPLPO.
The assay was designed as a duplex real-time PCR for the methylated SEPT9 y promoter and ACTB (actin, beta) as an internal reference to assess the integrity of each sample.