ACADM gene was expressed in all the adipose tissues and LD and the expression level in LD was higher than that in adipose tissues, though the difference was not statistically significant (Figure 4).
Comparison of PPAR[gamma], FASN, and ACADM mRNA expression levels between Yanbian yellow cattle and Yan yellow cattle in adipose tissue and LD
The mRNA expression levels of PPAR[gamma], FASN, and ACADM in adipose tissue and LD were compared between Yanbian and Yan yellow cattle (Figure 2, 3, and 4).
The relative expression level of ACADM gene in different adipose tissues and LD in two different cattle breeds is shown in Figure 4, but this difference was not statistically significant.
ACADM mRNA expression in other organs of Yanbian yellow cattle and Yan yellow cattle
ACADM mRNA levels were measured in the same samples in the two breeds (Figure 5C).
ACADM is the most important enzymes in the ACAD family which functions in the initial dehydrogenation step in the [beta]-oxidation of phenylbutyryl-CoA for C4 to C12 medium chain fatty acids (Illig et al.
In the present study, the ACADM gene was detected in most tissues, which may reflect its importance in energy production.
In summary, this study examined the expression levels of PPAR[gamma], FASN, and ACADM genes in different adipose tissues, LD, heart, liver, kidney, colon and lung in both Chinese Yanbian yellow cattle and Yan yellow cattle breeds, PPAR[gamma] and FASN mRNA expression levels were significantly higher in adipose tissues than in LD, which indicated that both genes play key roles in fat deposition.