Among those, we selected, for the microarray and RNA-seq analyses, four PG transporters, ABCC1 (also known as MRP1), ABCC9 (also known as sulfonylurea receptor 2 [SUR2]), SLCO4C1 (also known as OATP4C1), and SLCO5A1 (also known as OATP5A1), because these have been shown to be differentially expressed in the uterine endometrium during early pregnancy [10,11].
Although it has been shown that expression of ABCC1 gene is induced by estrogen and progesterone in human trophoblast cells , regulatory mechanisms of the expression of PG transporter genes, ABCC1, ABCC9, SLCO4C1, and SLCO5A1 are not well understood.
Therefore, to understand the expression and regulation of PG transporters in porcine uterine endometrium during the estrous cycle and pregnancy, we evaluated i) expression of ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs in the uterine endometrium during the estrous cycle and pregnancy, in the conceptus of early pregnancy, and in chorioallantoic tissues during pregnancy; ii) localization of ABCC1 and ABCC9 mRNAs in the uterine endometrium; and iii) effects of steroid hormones, IL1B, and interferon gamma (IFNG) on ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNA expression in the endometrial tissues.
Total RNA extraction and cloning of porcine ABCC1, ABCC9, SLCO4C1, and SLCO5A1 cDNAs
Experts found those with the gene ABCC9 need around 30 minutes more sleep per night than those without it.
When the researchers from the University of Edinburgh and Ludwig Maximilians University in Munich compared these figures with the results of the genetic analysis, they found those with a variation of a gene ABCC9 needed more sleep than the eight-hour average.
The gene ABCC9 is involved in sensing energy levels of cells in the body.
Karla Allebrandt and her team from the Ludwig Maximilians University of Munich identified a gene called ABCC9 that can reduce the length of time we sleep.
They discovered that people who had two copies of one common variant of ABCC9 slept for "significantly shorter" periods than people with two copies of another version.
Having already established that the ABCC9 gene was also present in fruitflies, the team were able to modify it in the animal and shorten the length of time for which it slept.