Quantitative real-time PCR was carried out as described below; briefly, total RNA was extracted, using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), from 2 x [10.sup.5] A375
cells for each experimental condition, according to the manufacturer's recommended procedure.
, WM35, SK-MEL-5, and SK-MEL-2 cell lines were obtained from cell bank of Wuhan university and incubated in DMEM added with 10% fetal bovine serum, 100 U/mL penicillin, and 100 [micro]g/mL streptomycin (Gibco, USA) in an atmosphere of 5% C[O.sub.2] at 37[degrees]C.
Proteomics analysis of' kojic acid treated A375
human malignant melanoma cells.
Wei et al., "Tubeimoside-1 inhibits growth via the induction of cell cycle arrest and apoptosis in human melanoma A375
cells," Bangladesh Journal of Pharmacology, vol.
In this study, we explored the cytotoxic and DNA damaging potential of PdNPs on human skin malignant melanoma (A375
Tsay, "Toxicogenomics of kojic acid on gene expression profiling of A375
human malignant melanoma cells," Biological and Pharmaceutical Bulletin, vol.
Human and rat melanoma cell lines were obtained from Bioresource Collection and Research Center (Taiwan): A375
(BCRC number 60039), A375
.S2 (BCRC number 60263), A2058 (BCRC number 60240), and B16-F10 (BCRC number 60031).
The other cryptotanshinone would restore the sensitivity of A375
melanoma cells that were resistant to TRAIL by upregulating the expression of death receptor 5 (DR5) .
Moreover, POL showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375
cells and induced apoptosis in a caspase-dependent manner.
To assess the biological function of NOP14 in melanoma cell lines A375
and SK-ML110, NOP14 overexpression and empty vectors were transiently transfected into cells.
Our finding are further supported by previous research reports where BHS-1 has been shown to inhibits STAT3 activation in A375
human melanoma, A431 human epidermoid carcinoma,U266 multiple myeloma and U937 acute myeloid leukemia cells (Khan et al., 2015).
134 - EXPRESSION OF NATIVE AND MUTATED ACVR1 IN TRANSIENTLY TRANSFECTED HUMAN A375