8-OHdG

8-OHdG

8-hydroxy-2’-deoxyguanosine. A modified DNA nucleoside product generated by reactive oxygen species (ROS), which is elevated in colorectal carcinoma, but not in adenomas. 8-OHdG is also elevated in ulcerative colitis (UC) and increases with disease duration and dysplasia. MUTYH, the human mutY homologue, is responsible for excising misincorporated adenine and for suppressing mutations caused by ROS. Nuclear expression of MUTYH is attenuated in UC.
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The amount of 8-OHdG in the DNA specimen was detected using an enzyme-linked immunosorbent assay kit (Cell Biolabs, Inc.
Results: SCI caused a significant decrease in tissue GSH levels and SOD activities, which were accompanied by significant increases in MDA and 8-OHdG levels and MPO and caspase-3 activities.
However, 14-day GTE supplementation significantly reduced oxidative DNA damage as measured by 8-OHdG after RE compared to PL (P < 0.
Results: No significant differences were observed between the patient group and controls in terms of age, high-density lipoprotein (HDL) cholesterol, 8-OHdG, F1+2 (p>0.
Em contrapartida, Rahimi (2011) apos administrar creatina durante 7 dias, em 27 homens submetidos ao exercicio resistido, constatou niveis inferiores do marcador urinario 8-OHdG (indicativo de lesao oxidativa no DNA) no grupo suplementado.
The human 8-oxoguanine DNA glycosylase 1 (hOGG1) and apurinic/apyrimidinic endonuclease 1 (APE1) genes play an important role in the DNA repair pathway [7] where hOGG1 has primary activity to remove the 8-OHdG from DNA through the BER mechanism whereas APE1 repairs basic AP sites in DNA.
Current evidence suggests that 8-OHdG lesions present in DNA during cellular replication results in somatic mutation, the driving force behind carcinogenesis.
5, 3, 6, and 12 ng/mL) and 40 [micro]L of sample DNA were incubated at 37[degrees] for 1 hour in the 96-well ELISA plate ready to use with capture antibody immobilized in the wells, with 10 [micro]L of 8-OHdG antibody and 50 [micro]L of streptavidin-horseradish peroxidase.
To evaluate the sperm DNA damage caused by ROS or oxidative stress, 8-OHdG and TUNEL assays were performed.
Changes in creatinine standardized F2-isoP (ng/mg creatinine) and 8-OHdG (lg/g creatinine) were not significant between groups at week 4.
Serum 8-OHdG levels were tested by enzyme-linked immunosorbent assay (ELISA) before and after treatment and the results in atorvastatin treatment groups were compared with the healthy controls.