Glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase
(6PGL), and 6-phosphogluconate dehydrogenase (6PGDH) are major proteins involved in the synthesis of NADPH and ribonucleotide through the oxidative branch.
In our previous study, hydroquinone, one of the benzene metabolites, induced significantly differential protein expression profile in human liver cells , and four proteins were identified, including Rho GDP dissociation inhibitor GDI alpha, 6-phosphogluconolactonase
, erbB3 binding protein EBP1, and lamin A/C.
Mass spectrometryanalysis of tissue extracts identified only 5 of the candidate biomarkers in the samples analyzed: apolipoprotein C1 (APOC1, gel 8), asporin (ASPN, gel 5), complement C4A (gel 8), 6-phosphogluconolactonase
(PGLS, gel 7), and ribosomal protein L22-like 1 (RPL22L1, gel 8).
Results of BLAST search performed with human EGF against the non redundant mycobacterial protein database at NCBI Accession Protein Name E-Value Number NP_302390 Probable multidrug resistance pump 0.022 NP_301513 GTP-binding protein LepA 0.40 NP_301554 Putative -alanyl-D-alanine carboxypeptidase 2.2 NP_302118 heat shock protein 90 2.4 NP_302586 UDP-N-acetylenolpyruvoylglucosamine reductase 4.4 NP_301491 6-phosphogluconolactonase
6.5 NP_301739 Possible ATP/GTP-binding protein 7.8 NP_301890 Mycocerosic acid synthase 8.8 Table 3.
The oxidative branch generates NADPH and ribonucleotides, with enzymatic regulation by glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase
(6PGL), and 6-phosphogluconate dehydrogenase (6PGDH).