Tenders are invited for Supply of 5-methylcytosine
(5-mC) monoclonal antibody, 5-hydroxymethylcytosine (5-hmC) monoclonal antibody (mouse)
Elsewhere, using an ELISA-like reaction with 5-methylcytosine
antibody, Zhang et al .
Global DNA methylation was assessed by determination of 5-methylcytosine
(5-mC) using ELISA-based MethylFlash[TM] Methylated DNA Quantification Kit (Epigentek) following the manufacturer's instructions.
Balasubramanian S: Quantitative sequencing of 5-methylcytosine
After fertilization, DNA demethylation is initiated via oxidation of 5-methylcytosine
(5mC) to 5-hydroxymethylcytosine (5hmC) mediated by enzymes of the ten-eleven translocation (TET1-3) family .
Chemical modification of DNA bases, 5-methylcytosine
, plays a key role in epigenetic gene regulation (Breiling and Lyko, 2015).
24) During DNA replication, DNMT1 reproduces 5-methylcytosine
(5mC) on newly synthesized DNA to maintain tissue- or cell-specific methylation patterns, so that dissociation of DNMT1 induces passive demethylation as cells divide.
Limited tissue culture-induced mutations and linked epigenetic modifications in F1 hybrids of sorghum pure lines are accompanied by increased transcription of DNA methyltransferases and 5-methylcytosine
The DNA-based approaches include sequencing and epigenetics based assays, which focus on the investigation of the methylation status of fetal DNA either using sodium bisulfite DNA treatment (7), methylation-sensitive restriction enzymes, or antibodies specific to the 5-methylcytosine
residues of CpG dinucleotides across the genome (8-10).
Hydrogen peroxide induced the formation of 5-methylcytosine
(5-mC), which is a cytosine variant produced by the transfer of a methyl group to the carbon located in the fifth position of cytosine.
Reprogramming of the paternal genome upon fertilization involves genome-wide oxidation of 5-methylcytosine
Functionally, they are a family of dioxygenases (TET 1/2/3), which convert 5-methylcytosine