DAPI

(redirected from 4',6-diamidino-2-phenylindole)
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DAPI

Abbreviation for 4'6-diamidino-2-phenylindole·2HCl. See: DAPI stain.
References in periodicals archive ?
Finally, to enable nuclear staining visualization, samples were incubated with 300nM of 4',6-diamidino-2-phenylindole (DAPI; Invitrogen) in PBS(1X) for 5 min at room temperature.
Cellular nuclei were stained by using 4',6-diamidino-2-phenylindole.
Finally, the cells were counterstained with 4',6-diamidino-2-phenylindole (DAPI) for 1-2 min, sealed with sealing liquid resistant to fluorescence quenching, and then examined under fluorescence microscopy (19).
Observation and scoring were performed using a Leitz Aristoplan Optical Microscope (Leica, Wetzlar, Germany), equipped with a fluorescence apparatus, with a triple bandpass filter for aqua, orange and green fluorochromes (Vysis) and a monochrome filter for 4',6-diamidino-2-phenylindole (DAPI, Vysis).
Nuclei were counter stained with 4',6-diamidino-2-phenylindole dihydrochloride hydrate (DAPI), washed extensively with PBS, and mounted with Vectashield antifade mounting solution (Vector Laboratories; Burlingame, Calif.
4) Representative images of soleus sections subjected to GLUT4 (green) and 4',6-diamidino-2-phenylindole (DAPI) (blue) immunofluorescence staining.
4',6-diamidino-2-phenylindole (DAPI) was used for nuclear counter stating of testis section.
Blocked eggs were then incubated >2 h to overnight in primary antibodies, followed by a 2-h incubation period in the appropriate fluorophore-conjugated, secondary antibodies, then mounted in ProLong antifade reagent containing 4',6-diamidino-2-phenylindole (DAP1; Thermo Scientific) in order to stain chromosomes.
Quantitative analysis of TdT-mediated dUTP-biotin nick end labeling (TUNEL; green) positive cells or 4',6-diamidino-2-phenylindole (DAPI; blue) to label all cells.
In a typical assay, the slides are stained with fluorescent antibodies to cytokeratin and CD45 and, for identification of DNA, 4',6-diamidino-2-phenylindole (DAPI).