DAPI

(redirected from 4',6-diamidino-2-phenylindole)
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DAPI

Abbreviation for 4'6-diamidino-2-phenylindole·2HCl. See: DAPI stain.
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Bone marrow paraffin sections of primary myelofibrosis (A through D), acute monocytic leukemia with reticulin myelofibrosis (E through H), multiple myeloma (I through L), chronic lymphocytic leukemia (M through P), and idiopathic thrombocytopenic purpura (Q through T) with 4',6-diamidino-2-phenylindole (DAPI) staining were exposed to 405-, 488-, 561-, and 633-nm channels in confocal laser scanning microscopy (original magnification X200).
The cells were rinsed 3 times with DPB[S.sup.++], and exposed to a 30-min incubation with a 1:3 dilution of 4',6-diamidino-2-phenylindole (DAPI) in DPB[S.sup.++].
Scheme of the experimental procedure for cell culture with seeding density of 1.5 x [10.sup.5] cells/scaffold (WST-8, PFA, GTA, and DAPI are, respectively, the viability assay, paraformaldehyde, glutaraldehyde, and 4',6-diamidino-2-phenylindole).
All migration assays were conducted at 37[degrees]C for 6 hours, and the cells were then fixed in 4% paraformaldehyde (PFA) and stained with 4',6-diamidino-2-phenylindole (DAPI; Roche Diagnostics, Indianapolis, IN, USA).
UltraCruz mounting medium with 4',6-diamidino-2-phenylindole (DAPI) was purchased from Santa Cruz.
The cells were then stained with 2.5mg x [L.sup.-1] of 4',6-diamidino-2-phenylindole (DAPI) for 10min and mounted with ProLong Gold antifade mounting medium.
The oocytes were rinsed with distilled water and stained in 8 [micro]L of 4',6-diamidino-2-phenylindole (DAPI) at a concentration of 2 [micro]g/mL (Barros e Silva & Guerra 2009).
Sections were stained for nuclei with 4',6-diamidino-2-phenylindole (DAPI, Beyotime, China), cover-slipped with fluorescent mounting medium (Beyotime, China), and observed under fluorescent microscope (Zeiss, Germany).
Mycelia were stained in 1% DAPI (4',6-diamidino-2-phenylindole) in dark condition for 5 minutes.
Cells were double-immunostained for Myc-STK3 (red) and FLAG-VP1 (green); cellular nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue).
Scale bar: 50 pm, 4',6-diamidino-2-phenylindole (DAPI): blue, cleaved PARP: green, Glu: D- glucose treatment for 24 hours, Let7A mimic: Let7A mimic pretreatment for 48 hrs, Let7A inhibitor: anti-Let7A pretreatment for 48 hrs, and CLD5: claudin 5.
Antifade mounting medium and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Biosscii Technology Co., Ltd.