3-mercaptopyruvate sulfurtransferase

3-·mer·cap·to·py·ru·vate sul·fur·trans·fer·ase

an enzyme that is a part of the cysteine catabolic pathway; it catalyzes the conversion of 3-mercaptopyruvate to pyruvate and H2S; a deficiency of this enzyme will result in elevated urine concentrations of 3-mercaptopyruvate as well as of 3-mercaptolactate, both in the form of disulfides with cysteine.
Mentioned in ?
References in periodicals archive ?
3-mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain.
Kimura, "3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathionepersulfide (Cys-SSH and GSSH) together with signaling molecules [H.sub.2][S.sub.2], [H.sub.2][S.sub.3] and [H.sub.2]S," Scientific Reports, vol.
Additionally, previous work has shown that 3-mercaptopyruvate sulfurtransferase in combination with cysteine aminotransferase can generate [H.sub.2]S from cysteine (23).
[H.sub.2]S is produced by cystathionine [beta]-synthase, cystathionine [gamma]-lyase, L-cysteine desulfhydrase, and 3-mercaptopyruvate sulfurtransferase (for an overview see [1] and references within).
It has a characteristic smell of rotten eggs and is produced in the mammalian cells through the enzymatic reactions of cystathionine [gamma]-lyase (CSE), cystathionine [beta]-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3MST).
To this end, we found that cells expressing 3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) contain increased levels of bound sulfane sulfur.
The gel was transferred to PVDF membranes (Millipore, MA, USA) and immunoblotted with antibodies: eNOS (1: 3000; Cell Signaling Tech., MA, USA), [peNOS.sup.S1177] (1:2000; Cell Signaling Tech.), AMPK (1: 3000; Cell Signaling Tech.), Akt (1: 2000; Cell Signaling Tech.), cystathionine-[gamma]-lyase (CSE, 1: 1000; Abnova, Taipei, Taiwan), cystathionine-[beta]-synthase (CBS, 1:1000; Abnova), and 3-mercaptopyruvate sulfurtransferase (3-MST, 1:1000; Abcam, Cambridge, UK).
Additionally, a role for 3-mercaptopyruvate sulfurtransferase in concert with cysteine aminotransferase has been identified in the vasculature [4].
Hydrogen sulfide ([H.sub.2]S) is an endogenous gaseous signaling molecule in mammalian tissues, which is enzymatically synthesized by cystathionine [beta]-synthase (CBS), cystathionine y-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST) [17-19].
Yoshida et al., "3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain," Antioxidants & Redox Signaling, vol.
Other enzymes such as 3-mercaptopyruvate sulfurtransferase (MST) and cysteine aminotransferase (CAT) are also important in specific tissue types [12].
These species contain orthologues of the mammalian [H.sub.2]S-generating enzymes cystathionine-[beta]-synthase (CBS), cystathionine-[gamma]-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3MST) and metabolize cysteine [15].