The mRNA expression of 3-ketoacyl-CoA thiolase
(3-KAT) was increased in diabetic hearts.
After the fourth step in which a 3-ketoacyl-CoA thiolase is involved, cycling continues until the final thiolytic cleavage with the production of two acetylCoA molecules.
The enzymes for the b-oxidation oflong- to mediumchain fatty acids (C-18 to C-12) are located close to the inner mitochondrial membrane, they are: very-longchain acyl-CoA dehydrogenase, long-chain enoyl-CoA hydratase, long-chain L 3-hydroxyacyl-CoA dehydrogenase and long-chain 3-ketoacyl-CoA thiolase.
The 3 enzymes involved are long-chain enoyl-CoA hydratase (LCEH), long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD), and long-chain 3-ketoacyl-CoA thiolase
1997) Medium-chain 3-ketoacyl-CoA thiolase
deficiency: a new disorder of mitochondrial fatty acid [beta]-oxidation.
The BLAST search analysis of the untransformed alfalfa sequence revealed 55 to 60% nucleotide identity with 3-ketoacyl-CoA thiolase genes from Arabidopsis (Hayashi et al.
cDNA cloning and expression of a gene for 3-ketoacyl-CoA thiolase in pumpkin cotyledons.
The long-chain forms of hydratase, LCHAD, and the 3-ketoacyl-CoA thiolase
exist as a multienzyme complex located in the inner mitochondrial membrane that is called the mitochondrial trifunctional protein (MTFP) (3, 4).
LCHAD activity resides in a membrane-bound multienzyme complex that also contains long-chain 3-enoyl-CoA hydratase and longchain 3-ketoacyl-CoA thiolase activities (trifunctional protein) [10,11].
The [beta]-subunit contains longchain 3-ketoacyl-CoA thiolase activity.