diaminobenzidine

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diaminobenzidine

(dī″ă-mēn″ō-bĕn′zĭ-dēn″),

DAB

A chromogen, C12H14N4, used in immunocytochemistry to highlight peroxidase.
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An ABC method (Vector Laboratories) was performed in combination with 3,3'-diaminobenzidine (Dojindo Laboratories, Japan) for antigen detection.
Endogenous peroxidase activity was blocked using 3% [H.sub.2][O.sub.2] diluted in methanol, reagents were applied manually, and the immunoreactivity was visualized by 3,3'-diaminobenzidine (Lab Vision DAB substrate system; Lab Vision, Fremont, California, USA).
Antibody-antibody-conjugate complexes were detected with an enzyme substrate comprising 3,3'-Diaminobenzidine.
enhancer Abbreviations: Ab, antibody; CC, cell condition; DAB, 3,3'-diaminobenzidine; GtAMs, goat anti-mouse; GtARb, goat anti-rabbit; HIER, heat-induced epitope retrieval; IgG1, immunoglobulin G1; Phe, phenylalanine; Thr, threonine; TRS, target retrieval solution.
Pretreatment using a heat-induced epitope retrieval (HIER) procedure prior to IHC was performed and DAB (3,3'-diaminobenzidine tetrahydrochloride) was used as a chromogen.
Al dia siguiente se efectuo la incubacion con el anticuerpo secundario biotinilado (AM-B1-UNL) en dilucion 1/100 a temperatura ambiente por 30 min y luego exposicion de 30 min a extravidina (Sigma) en dilucion 1/200 y solucion DAB (3,3'-diaminobenzidine) durante 1 min.
Antigen-antibody complexes were detected by the avidin-biotin-peroxidase method with 3,3'-diaminobenzidine tetrahydrochloride.
staining was performed with bond polymer detection kit and ultra view 3,3'-diaminobenzidine (DAB) detection kit and the sections were developed with diaminobenzidine tetrahydrochloride and counterstained with hematoxylin.
Each slice was then stained with 3,3'-diaminobenzidine staining, restained with hematoxylin and eosin dehydrated with graded alcohol and mounted using neutral gum.
Ltd.; Beijing, China) for 30 min followed by 3,3'-diaminobenzidine (DAB) and hematoxylin staining, respectively.
Caption: Figure 1: SERT expression is specific and sensitive to the lung microvasculature (3,3'-diaminobenzidine, SERT antibody expression in brown).