Antigen-antibody complexes were detected by the avidin-biotin-peroxidase method with 3,3'-diaminobenzidine
staining was performed with bond polymer detection kit and ultra view 3,3'-diaminobenzidine
(DAB) detection kit and the sections were developed with diaminobenzidine tetrahydrochloride and counterstained with hematoxylin.
Subsequently, sections were incubated with 3,3'-diaminobenzidine
(DAB) and counterstained with hematoxylin (Mayer).
Each slice was then stained with 3,3'-diaminobenzidine
staining, restained with hematoxylin and eosin dehydrated with graded alcohol and mounted using neutral gum.
After developing with 100 mg of 3,3'-diaminobenzidine
(D-5637, Sigma, St.
Slides were incubated with the primary antibody for 15 minutes, with postprimary polymer for 8 minutes, and were blocked with 3% hydrogen peroxide for 5 minutes and 3,3'-diaminobenzidine
(brown chromogen) for 10 minutes, followed by hematoxylin counterstain for 5 minutes.
In the present work, PB1 containing a large number of ether linkages was prepared with 3,3'-diaminobenzidine
and 4,4'oxybis (benzoic acid) by the direct polycondensation in polyphosphoric acid to enhance the flexibility and improve the solubility.
Paraffin-embedded sections were prepared for immunohistochemistry using 3,3'-diaminobenzidine
(DAB) as described in "Methods.
MA1-38223), which was developed with 3,3'-diaminobenzidine
For the most part, the vast majority of microscopic pathology is still done by viewing or imaging one marker at a time in serial sections, using a hematoxylin counterstain and either eosin (H&E) or, for protein IF1C measurements, 3,3'-diaminobenzidine
(DAB) as a chromogen.
The sections were again washed in three drops of PBS and revealed by treatment with 3,3'-diaminobenzidine
tetrahydrochloride (003222--DakoCytomation, California, USA) prepared according to the kit instructions, washed in distilled water, dehydrated in an increasing concentration series of ethanol solutions and mounted using Entellan (Merck).
Immunostaining was developed by using chromogen, 3,3'-diaminobenzidine
and counterstained with Mayer's hematoxylin.