One of the selection strategies in mutagenesis is the use of simple sugar like glucose and non-metabolizable analogs like 2-deoxyglucose
(2-DG), it increases the enzyme activity and provides feedback resistance .
uptake assay was performed as described previously .
as an Energy Restriction Mimetic Agent: Effects on Mammary Carcinogenesis and on Mammary Tumor Cell Growth in vitro.
1: Experimental conditions, medium composition, type of diabetes emulated, and group abbreviations used in this study Experimental R16 medium composition Diabetes condition Control 19 mM glucose = 2 [micro]g/ml insulin Osmotic control 19 mM glucose 2 [micro]g/ml insulin 15 mM mannitol (4 days) No insulin 19 mM glucose Type 1 0 [micro]g/ml insulin (4 days) High glucose, 34 mM glucose (4 days) Type 1 no insulin 0 [micro]g/ml insulin (4 days) High glucose 34 mM glucose (4 days) Type 2 2 [micro]g/ml insulin 2-deoxyglucose
19 mM glucose Type 2 2 [micro]g/ml insulin 20 mM 2-deoxyglucose
(24 h) Experimental Abbreviation condition Control Control Osmotic control Mannitol No insulin No INS High glucose, HG, No INS no insulin High glucose HG 2-deoxyglucose
58) The reprogramming toward glycolytic metabolism seems to be a prerequisite for Q-HSC to A-HSC differentiation for the following reasons: first, addition of 2-deoxyglucose
, an inhibitor of glycolysis, to HSC cultures resulted in decreased proliferation, repressed expression of A-HSC genes, re-expression of lipogenic genes, and re-accumulation of lipids.
G]-monomethyl-L-arginine monoacetate (L-NMMA), significantly decreased both the basal 2-deoxyglucose
transport and NO efflux.
The compound also reportedly enhanced uptake of 2-deoxyglucose
dose-dependently in L6 myotubes and caused increased expression of glucose transporter type 4 (GLUT4) and phosphoinositide 3-kinase (PI3K) genes suggesting that the compound can replace commercially available anti-diabetic drugs (Prabhakar and Doble, 2010).
MU: In your book you mention 2-deoxyglucose
as an adjunctive treatment to the CRKD.
Interestingly, uptake of 2-deoxyglucose
occurs so fast that it competitively inhibits the transport of glucose.
Use of radiolabeled 2-deoxyglucose
may affect the suitability of this assay in a screening context.
Targeting cancer cell metabolism: the combination of metformin and 2-deoxyglucose
induces p53-dependent apoptosis in prostate cancer cells.
Phosphorylation and 14-3-3 binding of Arabidopsis trehalose- phosphate synthase response to 2-deoxyglucose