(2DG) acts as an inhibitor of glucose metabolism since it inhibits hexokinase, which is the first limiting factor enzyme of glycolysis.
Sodium azide (Na[N.sub.3]), potassium cyanide (KCN), sodium fluoride (NaF), ATPase inhibitor bafilomycin A1, or mixture of Na[N.sub.3] with 2-deoxyglucose
does not affect half-times and size of fast cholesterol pool in CHO-K1 cells or just slightly affects them in CHO cells expressing human transferrin receptor [14, 28].
Transport experiments were initiated by replacing the buffer with 100 [micro]L HEPES buffer (pH 7.4 and glucose free), containing [[sup.3]H] 2-deoxyglucose
(5mM in glucose-free HEPES buffer) and wheat extracts.
[sup.18]F-Fluoro-2-deoxyglucose (FDG), a nonmetabolizable 2-deoxyglucose
analogue, blocks glycolysis and inhibits protein glycosylation .
Lee, "ANG II increases 2-deoxyglucose
uptake in mouse embryonic stem cells," Life Sciences, vol.
In such a context, the first strategy which can be adopted consists in the employment of glucose analogs such as 2-deoxyglucose
, which enter the cell via glucose transporters and are phosphorylated by hexokinase, which cannot be further metabolized.
Scheich, "Alteration of auditory cortex activity with a visual stimulus through conditioning: a 2-deoxyglucose
analysis," Neurobiology of Learning and Memory, vol.
Indeed, the pathways involved in the production of cellular energy were more activated in T cells from SLE patients and lupus-prone mice, and the metabolic inhibitors metformin and 2-deoxyglucose
reduced disease severity in murine lupus [39-41].
The mutagenized conidia were grown in liquid minimal medium having 2% xylose and 0.5% (w/v) 2-deoxyglucose
The cannabinoid CB1 receptor antagonist rimonabant stimulates 2-deoxyglucose
uptake in skeletal muscle cells by regulating the expression of phosphatidylinositol-3-kinase.
Changes in glucose metabolism alter the seizure susceptibility and are evident in FDP, KD, and 2-deoxyglucose
(2DG), a compound acting on glucose uptake.
Interestingly, it has been recently shown that inhibition of aerobic glycolysis using the hexokinase inhibitor 2-deoxyglucose
improves the antiproliferative effect of metformin, a well-known inhibitor of mitochondrial electron transport chain, in TC cell lines.