ribosomal RNA

(redirected from 16s gene)
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RNA

 
messenger RNA (mRNA) see ribonucleic acid.
ribosomal RNA (rRNA) see ribonucleic acid.
transfer RNA (tRNA) see ribonucleic acid.

ri·bo·som·al RNA

the RNA of ribosomes and polyribosomes.

ribosomal RNA

n. Abbr. rRNA
The RNA that is a permanent structural part of a ribosome.

ribosomal RNA

Molecular biology Any of a family of single-stranded nucleic acids ranging from 100 to 3000 bases in length, that assemble in heteromultimeric complexes with proteins, to form ribosomes, the 'docking stations' for mRNA and nascent polypeptide strands. See Ribosome, RNA. Cf mRNA, tRNA.

ribosomal RNA (rRNA)

Ribonucleic acid that is a permanent structural feature of RIBOSOMES.

ribosomal RNA (rRNA)

a form of RNA transcribed from DNA in the NUCLEOLI of EUKARYOTE cells or clustered ribosomal genes in PROKARYOTES, that complexes with various proteins (r proteins) to form the RIBOSOME. rRNA constitutes the major proportion of RNA in cells and occurs in a number of different types (for example, in bacterial ribosomes: 23S rRNA, 16S rRNA and 5S rRNA). In protein synthesis a sequence near the 3′-end of the 16S rRNA interacts with the SHINE-DALGARNO sequence to initiate TRANSLATION. Certain regions of rRNA have been highly conserved during evolution and comparative sequencing studies of rRNAs are used to determine evolutionary relationships between organisms and to construct PHYLOGENETIC TREES.
References in periodicals archive ?
Because 16S sequencing relies on PCR amplification of the 16S gene, it is subject to the inherent biases of PCR.
This is interesting, as the most abundant rRNA 16S gene sequences also clustered close to this organism (Figure 4).
This was especially important in the case of the 16S gene, which presented a large number of gaps when comparing sequences of the most divergent species.
We conducted a nucleotide BLAST search (BLASTn) of the unknown frog 16S gene on Genbank.
In vitro amplification of the sequences belonging to the rRNA 16S gene was conducted on the thermal cycler (Thermal Cycler, Veriti[R] 96-Well--Applied Biosystems).
More than 170 zebra mussel bacterial 16S gene sequences were checked for similarity to 16S sequences contained in the Ribosomal Database Project and BLAST public databases.
rDNA 16S gene amplification from Klebsiella genus species were performed under the following reaction conditions: Klebrib-1 (5'-GTAATGTCTGGG AAACTGCC-3') [0.5 [micro]M] and Klebrib-2A (3'-CCACC TTCCTCCAGTTTATC-5') [0.5 [micro]M] Taq polymerase [0.25U], MgCl [1.5 mM], dNTPs [0.2 mM], PCR buffer [1X] and DNA sample [10 ng/[micro]l], adjusted to a final volume of 50 [micro]l.
Amplification primers 5'-CCTAACACATGCAAGTCGARCG-3' (forward) and 5'-CGTAT-TACCGCGGCTGCT-3' (reverse), both from Eurogentec (Seraing, Belgium) were used in a standard polymerase chain reaction (PCR) to generate a 490-bp fragment from the 5' end of the 16S gene. The PCR (25 [micro]L) consisted of 1 [micro]L DNA, 0.5 [micro]mol/L of both PCR primers, 1.5 mmol/L Mg[Cl.sub.2], 0.2 mmol/L dNTP, and 1 U FastStart Taq DNA polymerase (Roche Diagnostics, Almere, the Netherlands) in 1 x reaction buffer.
anthracis had an identical 16S gene sequence, designated type 6; 16S type 10 was seen in all B.
However, despite the similarities, the 16S gene sequence of M175 differed from M.
According to the results from the BLAST search (Table III), the sequences of COI and 16S genes for the AF matched Diporochaeta sp., because the only a few studies have been published concerning DNA in E.