This was especially important in the case of the 16S gene, which presented a large number of gaps when comparing sequences of the most divergent species.
The optimum models of substitution selected by jModelTest were TIM3 + G for the 16S gene (for both ML and BI), whereas for COI, different models were selected for ML (GTR + G) and BI (TIM2 + G) for the unpartitioned data set, and TIM 2 + I + G for the codon partitioned data set.
We conducted a nucleotide BLAST search (BLASTn) of the unknown frog 16S gene
A simple algorithm for specific differentiation at subspecies level with single rDNA 16S gene digestions for K.
rhinoscleromatis was tested in silico through gene pattern prediction of 759 bp and 311 bp of a sequence rDNA 16S gene with Sal I restriction enzyme (Figure 2A); nevertheless, due to polymorphism described among the ADNr 16S gene for K.
Polymerase chain reaction (PCR) amplification of the bacterial 16S gene
was performed using the universal eubacterial primer set 27f-1387r (27f: 5'-AGA GTT TGA TCM TGG CTC AG-3') and 1387r (5'-GGG CGG WGT GTA CAA GGC-3') (Marchesi et al.
Table 1 Reported sequences of rDNA 16S gene
for Klebsiella species included in this study Bacterial species Accession Source/Reference number Klebsiella pneumoniae AF228918 Genbank/ATCC13886 pneumoniae Klebsiella pneumoniae Y17656 Genbank/ATCC1383T Klebsiella pneumoniae AF228920 Genbank/NLEP98-0472 Klebsiella pneumoniae X93214 Genbank/DSM30102 Klebsiella pneumoniae AB004753 Genbank/JCM1662 Klebsiella pneumoniae AF130982 Genbank/ATCC:11296 ozaenae Klebsiella pneumoniae AF228919 Genbank/ATCC13885 ozaenae Klebsiella pneumoniae Y17654 Genbank/ATCC11296T ozaenae Klebsiella pneumoniae AF130983 Genbank/ATCC:13884 rhinoscleromatis Klebsiella pneumoniae AF009169 Genbank/NCTC5046 rhinoscleromatis Klebsiella oxytoca Y17655 Genbank/ATCC13182T Klebsiella granulomatis AF010251 Genbank/KH22
anthracis had an identical 16S gene
sequence, designated type 6; 16S type 10 was seen in all B.
However, despite the similarities, the 16S gene
sequence of M175 differed from M.
According to the results from the BLAST search (Table III), the sequences of COI and 16S genes for the AF matched Diporochaeta sp.
The nucleotide sequence of COI and 16S genes for the red worm (P.
In previous studies we analyzed the mitochondrial COI and 16S genes
of octocorallians and have found abnormally low levels of variation among them.