sequencing: Sequencing of 16S rDNA
of selected strains of each species of Lactobacillus was carried out at the National JALMA Institute for Leprosy and other Mycobacterial Diseases, Agra.
The DNA samples (obtained using the 16s rDNA
V3 primer) were sent to Germany (MGW-Biotech, Germany) for nucleotide sequencing.
Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA
Whereas 16S rDNA
sequences are highly conserved, the genome of some bacterial species harbors multiple copies of the ribosomal operon.
pneumoniae was obtained by 16S rDNA
PCR alone in 17 of the 40 cases (43%), all of whom had received antibiotics prior to pleural fluid aspiration.
Given that microbial identification of such environmental isolates is problematic with rapid-identification techniques based on biochemical gallery strips--mainly because of a lack of biochemical profiles of these organisms--it was decided that all identifications should be performed with broad-range or universal 16S rDNA
, as previously done in the identification of environmental organisms (Moore et al.
PM1 is a gram-negative rod and, based on 16S rDNA
sequence similarity, is a member of the beta subgroup of Proteobacteria, in the family Comamonadaceae, and closely related to Aquabacterium, Rubrivivax, Leptothrix, Ideonella, and Hydrogenophaga (Bruns et al.
The infection of P acnes in the present case was evident by the botryomycotic granules and amplification of 16S rDNA
PE Biosystems also introduced three products: the MicroSeq 500 16S rDNA
Bacterial Sequencing Kit that provides higher sample throughput, faster results, and lower reagent costs; and two kits that make detection of food-borne E.
The sequencing of 16S rDNA
of the strain SBF1 was done commercially by DNA Sequencing Service, Macrogen, Inc.
Traditional cultural method and the 16S rDNA
analysis were used to identify the bacterial genus and the bacterial quantities.