of midgut proteins from Trichoplusia ni with either 2% Xen-Tari (A) or 1% Cry1Ac-HD73 (B) as substrate.
were performed according to the procedure of Laemmli (1970) except that samples were not heated and no reducing agents were added.
Recovered samples were loaded individually onto a phenyl-column, eluted with equilibration buffer without ammonium sulfate, and analyzed by zymogram
Fungal proteins with mannanase activity identified directly from a Congo Red stained zymogram
studies revealed an amylase active band for both fungi with an approximate molecular weight of 67 kDa.
The supernatants were detected in duplicate with a Gelatin Zymogram
Assay Kit (Applygen Technologies, Beijing, China) for MMP-9 activities according to the manufacturer's instructions.
The graph above the zymogram
shows the lack of inhibition of proliferation (MTS assay) at this time point.
1984) were followed for the zymogram
analysis of concentrated conditioned media derived from OSM-treated mammary carcinoma cell cultures demonstrates OSM-induced up-regulation of several proteases, including matrix metalloproteinase (MMP)-7, MMP-9, and MMP-13.
interpretation was achieved following recommendations by Utter et.
5% acid swollen chitin was used as substrate for chitinase in the zymogram
Maize root revealed aldehyde oxidase (AO) activities in the zymogram