Ferrous ion oxidation in the presence of ferric ion indicator xylenol
orange for measurement of hydroperoxides.
Jiang, ZY, Hunt, JV, Wolff, SP, "Ferrous Ion Oxidation in the Presence of Xylenol
Orange for Detection of Lipid Hydroperoxide in Low-density-lipoprotein.
Lipid hydroperoxides (LHP) were detected by their ability to oxidize ferrous ion under acidic condition in the presence of xylenol
orange resulting in the formation of a chromophore with absorption maxima at 560 nm (13).
2+] at a low pH in the presence of the dye xylenol
orange (XO) and the amount of [Fe.
Quantification of lipid peroxidation in tissue extracts based on Fe(III) xylenol
orange complex formation.
The ferric ion generates a colored complex with Xylenol
Orange in an acidic medium.
2+] was determined in the presence of xylenol
orange in the supernatant by measuring the absorption at 540 nm, Standard solutions of [H.
Supernatant samples (100 [micro]L) were incubated for 30 min with 1 mL of aqueous peroxide color reagent (aqueous solution containing 100 mM sorbitol and 125 [micro]M xylenol
orange) and 10 [micro]L of ferrous ammonium sulfate reagent (25 mM ferrous ammonium sulfate in 2.
Of the three chemical ingredients specified by WHO, EcoTru(R)'s exclusive technology is able to deliver chloro meta xylenol
at an extremely low concentration, creating a powerful hospital grade disinfectant that is non-hazardous and environmentally safe.
An aliquot of tissue homogenate or mitochondria (100 [micro]g protein) was added to 1 ml FOX reagent (100 [micro]M xylenol
orange; 250 [micro]M ammonium ferrous sulphate; 100;[micro]M sorbitol; 25 mM [H.
The ferric ion produced a colored complex with xylenol
orange in acidic solution.