Wet preparations of the motile fractions were prepared by removing a 10 [micro]l aliquot, which was placed onto a clean glass slide and covered with a 22 x 22 mm coverslip.
This study aimed to develop a simplified method to evaluate the sperm concentration using an office-based microscope to record sperm concentration by estimating the number of sperm on a standardised wet preparation.
In cases where >40-100 and >100 sperm were observed on the wet preparation, a 1:20 dilution was used, while a 1:2 dilution was used for counts [less than or equal to] 40.
The results, portrayed in fastidiously realistic re-creations of accidents, complete with wax models of seared flesh and wet preparations
of actual limbs, are not pretty.