This idea is further supported by the detection of p58 in the cytoplasm of all four blastomeres of four-celled ME embryos produced from the vegetal pole region of fertilized eggs [ILLUSTRATION FOR FIGURE 14 OMITTED].
During the first phase of ooplasmic segregation following fertilization, the MCD is moved towards the vegetal pole.
In contrast, nearly the entire cortical cytoplasm of smaller ME fragments produced from the vegetal pole region of fertilized eggs contained the MCD [ILLUSTRATION FOR FIGURE 14C OMITTED].
Localization of axial determinants in the vegetal pole region of ascidian eggs.
The parallel organization of the microtubule network near the vegetal pole was lost by this stage [ILLUSTRATION FOR FIGURE 2C OMITTED].
The mat of parallel microtubules at the vegetal pole of the medaka egg is reminiscent of the one found at the vegetal pole of amphibian eggs, where it is involved in the cortical rotation that forms the gray crescent and thus determines the dorsal-ventral axis of the embryo (Manes et al.
Because microtubule poisons inhibit the movement of oil droplets toward the vegetal pole of the medaka egg, it has been suggested that these droplets are transported along microtubules by microtubule-based motors (Abraham et al.
The movement of most oil droplets toward the vegetal pole of the medaka egg begins at [T.
CCD had no apparent effect on the segregation of oil droplets toward the vegetal pole [ILLUSTRATION FOR FIGURE 1B OMITTED] or on the saltatory movement of particles toward either the animal or vegetal pole.
1993a): (1) oil droplets did not segregate to the vegetal pole but instead floated to the portion of the egg that was uppermost during the experiment - equator [ILLUSTRATION FOR FIGURE 1C OMITTED], animal pole [ILLUSTRATION FOR FIGURE 2C AND D OMITTED], or vegetal pole; (2) a smaller-than-normal blastodisc formed [ILLUSTRATION FOR FIGURE 1C OMITTED]; (3) saltatory motion was absent; and (4) the eggs did not cleave.
In eggs treated with both CCD and demecolcine, we saw no saltatory movement, no cytoplasmic streaming, and no movement of oil droplets toward the vegetal pole [ILLUSTRATION FOR FIGURE 1D OMITTED].