Release of an active molecule from its inert protective enclosure at a chosen locus.
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0) and in situ manipulation, option and thus suitability for electrophysiological experiments, suitable for optoelectronic techniques (stimulation, inhibition, Uncaging, Unmixing YFP vs.
Scientifica's LASU is specifically suited to enhance the combination of electrophysiology, optogenetics and uncaging for advanced studies of neuronal processes.
A second synchronized beam at 1041 nm is available for advanced imaging techniques, including uncaging, dual-wavelength simultaneous imaging, second-harmonic generation (SHG), third-harmonic generation (THG), coherent anti-Stokes Raman scattering (CARS), and stimulated Raman scattering (SRS) imaging.
For this second edition, he updates standard techniques and introduces three new technical advances in the field: two-photon lasers and uncaging experiments, in vivo techniques, and high throughput techniques using planar patch electrodes for pharmacological testing.
Columbia TriStar is uncaging "Animal" June 1 in the U.
The Cornell researchers say their method could help solve a targeting problem encountered with uncaging these molecules, because only the chromophore in the bright focus of the laser will be activated.
Their activation will be induced using different levels and patterns with high resolution glutamate uncaging, in adjacent or remote spines including on different dendritic branches.