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The cutting of ultrathin sections for electron microscopy by use of an ultramicrotome.
References in periodicals archive ?
Although hard minerals can be successfully sectioned by ultramicrotomy using a diamond knife, damage to the knife can easily occur, making the technique costly.
Samples are prepared by ultramicrotomy, but require no labeling of the coating components.
The block faces of specimens after cryo ultramicrotomy were investigated at ambient conditions using ZEISS Axioplan light microscope (ZEISS, Germany) equipped with ZEISS Axio Cam ICc I CCD camera in reflected polarized light, and by Dimension 3100 AFM/SPM (Veeco, USA) atomic force microscope.
The ultramicrotomy is performed in a Leica EM UC7FC7 microtome at the temperature of -80[degrees]C, first with a Diatome cryotrim in order to withdraw several tens of micrometers that could be damaged by the blade and then the ultrathin sections of about 60-80 nm in thickness are cut using a Diatome diamond knife of 35[degrees] with a cutting speed in the range 0.
The ultrathin sections of sample with the thickness 70-80 um were prepared via ultramicrotomy technique using Reichert Ultramicrotome Supernova.