The embedded samples were sectioned at 500 nm thick with a Power Tome PC (RMC Products) Ultramicrotome
, dyed with Touluidin Blue and observed with an Olympus IX-51 inverted light microscope.
Afterwards, the blocks were shaped as trapezes, having 1 mm on each side and then sliced into sections using an ultramicrotome
Ultra-thin 90 nm thick cross sections were cut with a Porter Blum MT2 ultramicrotome
, the sections were stained with uranyl acetate and lead citrate for observation under transmission electron microscopy.
85 [micro]m) and ultra-thin (<100 nm) using a diamond knife in a Reichrt-jung ultramicrotome
Thin sections were cut with an ultramicrotome
and diamond knife and stained with lead citrate.
5 to 1 mm thick were cut with a Reichert Ultracut E ultramicrotome
and placed onto microscope slides before being stained with toluidine blue.
Thin sections of 100 mm were cut in a Reichert Om U3 ultramicrotome
and counterstained with uranyl acetate (45 min, 60[degrees]C) and lead citrate (3 min, 25[degrees]C).
5 [micro]m sections were made on Reichert-Jung (Ultracut E) ultramicrotome
with a glass knife, for the localization of fungal structures of interest in a light microscope and for orientation of the ultrathin sections.
Thin sections of selected areas of the epoxy block were cut with an ultramicrotome
using a diamond knife.
For transmission electron microscopy (TEM) measurements, 100 nm sections were microtomed at -120[degrees]C using Ultracut E ultramicrotome
(Reichert and Jung) with a diamond knife.
Ultrathin sections of the films were cut on an ultramicrotome
with cryo-attachment (Ultracut UCT, Leica) at--110[degrees]C.
Ultrathin sections of the fibres (50--70 nm) were cut by an ultramicrotome
(Leica Ultracut UCT) using diamond knife (Diatome, Leica), stained with Uranyl acetate (EM Sciences)--Lead citrate (Ladd Chemicals, USA) and imaged under the microscope (Hitachi H-600).