The Leap-In Transposase
rapidly catalyzes the stable integration of genes into a target genome.
In the study, researchers tagged cells using a transposon, a piece of genetic code that can jump to a random point in DNA when exposed to an enzyme called transposase
, to track blood progenitors and adult stem cells during the natural, unperturbed process of blood regeneration.
Assay for Transposase
Accessible Chromatin (ATAC) Sequencing and Data Processing
Recently, an efficient transgenic production system was established using piggyBac transposase
and transposon elements [2,3].
In comparing the genetic context of rmtE1 and rmtE2, ISCR20-like transposase
is located upstream of rmtE1 and rmtE2 (GenBank accession nos.
cpl] 26 transposase
ISEcp1R TTTCCGCAGCACCGTTTGC [ISE.
A transposon is a piece of genetic code that can jump to a random point in DNA when exposed to an enzyme called transposase
Virtually all marin-like elements found in natural populations are non-functional pseudogenes, containing stop signals, deletions, frameshifts, or missense mutations that either disrupt the open reading frame or produce an inactive transposase
The company tested more than two million variations of the piggyBacTM transposase
enzyme and discovered versions of the enzyme that are more than 10 times more active than the version found in nature.
This technology uses a transposase
enzyme complexed with a transposon, which, in this case, contains Illumina adapters (product data sheet).
The PiggyBac process involves shipping cargos of genetic material - called transposons - around the genome using an engine known as a transposase
They possess an enzyme called a transposase
which enables them to cut-and-paste themselves into new sites in the genome.