transfection

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trans·fec·tion

(trans-fek'shŭn),
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

(trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.

trans·fect′ v.

transfection

[-fek′shən]
Etymology: L, trans + inficere, to taint
the process by which a bacterial cell is infected with purified DNA or RNA isolated from a virus after a specific pretreatment. Acute transfection is short-term infection. transfect, v.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

Gene transfer by infection of a cell with nucleic acid by a virus, followed by viral replication in the affected cell.

transfection

a method of introducing the purified DNA of a VIRUS into CELLS.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

an introduction of free DNA into a cell.
References in periodicals archive ?
Nowadays, Mirus Bio is the leading manufacturer of transfection reagents for chemical and/or electroporation delivery of DNA, RNA, siRNA, miRNA, and other nucleic acids.
Thereby, the optimization of the TGE technique for Chinese hamster ovary (CHO) as the dominant host for the production of biotherapeutics is of great interest to reach the values for Human Embryo Kidney-293 (HER-293) cells in terms of transfection efficiencies and production titers.
A 5 | g transfection of a GFP-expressing plasmid served as a negative control.
In this study, we tried to optimize transfection of MSCs with GFP containing phGFP vector and then examined the fate of MSCs both in vitro and in vivo.
Transfection of Gametes: A Method to Generate Transgenic Animals
In a trial using CMV-LUC (4 [micro]L of 500 ng DNA/pL; n = 5), approximately 80-90% of the total brain luciferase activity was found in the telencephalon and optic tectum, whereas the hypothalamus and cerebellum plus hindbrain had very low levels of transfection (data not shown).
The presence of blue-stained cells after 24 hours indicated positive transfection.
RmesFect[TM] and RmesFect[TM] Stem have been developed for very efficient transfections of mRNA in a wide variety of immortalized and primary cells.