transfection

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trans·fec·tion

(trans-fek'shŭn),
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

(trăns-fĕk′shən)
n.
Introduction of a segment of DNA or RNA into a eukaryotic cell by means of one of a variety of physical or chemical methods or through viral infection.

trans·fect′ v.

transfection

[-fek′shən]
Etymology: L, trans + inficere, to taint
the process by which a bacterial cell is infected with purified DNA or RNA isolated from a virus after a specific pretreatment. Acute transfection is short-term infection. transfect, v.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection

Gene transfer by infection of a cell with nucleic acid by a virus, followed by viral replication in the affected cell.

transfection

a method of introducing the purified DNA of a VIRUS into CELLS.

trans·fec·tion

(trans-fek'shŭn)
A method of gene transfer using infection of a cell with nucleic acid (as from a retrovirus) resulting in subsequent viral replication in the transfected cell.
[trans- + infection]

transfection (transfek´shən),

n the process by which a bacterial cell is infected with purified deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) isolated from a virus or a viral vector after a specific pretreatment.

transfection

an introduction of free DNA into a cell.
References in periodicals archive ?
This is particularly valuable for stem cells and primary cells that are the most medically relevant and the most difficult to transfect.
The X-tremeGENE 9 reagent offers efficient performance across a wide variety of commonly used cell lines while Roche's X-tremeGENE HP reagent is especially well suited for cell lines that are difficult to transfect or not easily transfected with other reagents.
Broad Spectrum DNA Delivery--Achieve high transgene expression in many cell types, including hard to transfect cells.
In addition to demonstrating the simple operation of the MaxCyte STX to transfect any cell with any molecule at any scale quickly and reproducibly, Dr.
The MaxCyte STX represents a fast, simple, and scalable way to transfect cell lines, primary cells, and stem cells with high efficiency and performance for cell-based assays for screening ion channels, GPCRs, and other targets," says Dr.
Reardon reviewed the complexities of cell transfection technologies and challenged the webinar participants to consider the possibilities of creating more relevant assays if it were possible to transfect any molecule into any cell at any scale, while maintaining biological integrity and high quality standards.
The ability of the MaxCyte STX to transfect cells with ion channels and other hard-to-transfect targets is proving to be a valuable tool for pharmaceutical companies to accelerate the screening of compound libraries for drug candidate selection.
Madhusudan Peshwa, Vice President of Research and Development at MaxCyte, "The MaxCyte STX Scalable Transfection System can be used to transfect primary cells, cell lines, and stem cells with a range of molecules, including DNA plasmids, different types of RNA, proteins, etc.
Lipid-based reagents are typically used to transfect siRNAs into immortalized cell lines, but tend to be inefficient to deliver siRNA to most primary cell types.
James Brady, Director of Technical Applications, will be making a technical presentation on Wednesday, April 29 at 10:10 AM, titled "Scalable Electroporation System Transfects Large Volumes of Cells for Cell-Based Assays & Other Drug Screening Applications.