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The difference in transconjugant colony count (TCC) between control and test was noted.
Results The use of the chloramphenicol antibiotic and the calcofluor white as selection agents have made it possible to select transconjugant strains capable to produce the exopolysaccharides.
Transconjugant was selected on combined plates containing meropenem (1 mg/L) to inhibit the growth of recipient strain and rifampicin (256 mg/L) or sodium azide (100 mg/L) to suppress donor strains.
Putative transconjugant colonies were selected and identified by the Vitek system, and further confirmed in a [bla.
Characterization of a series of transconjugant mutants of an Avian Pathogenic Escherichia coli isolate for resistance to serum complement.
Soybean transformations were conducted with the resultant transconjugant as previously described (Zhang et al.
0] by mixing each strain with a donor and selecting a transconjugant after overnight matings.
EMB agar in order to correlate the effect of the transconjugant detection technique with the occurence at transconjugant (plate-mating) [22].
Transconjugant presence was demonstrated by culture turbidity and medium pH indicator color change under selective conditions.
The MIC of colistin for the transconjugant (4 mg/L) was 8 times higher than that for E.
The transconjugant (TC-J53) also showed resistance to ampicillin, erythromycin streptomycin, ceftriaxone, cefotaxime, cefuroxime, and imipenem (MIC 2 mg/L), indicating the possibility that the NDM-1 plasmid also harbored genes encoding resistance to these antibiotics.
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