Currently, there are no studies about another characteristics different to morphometric parameters, like chromatin integrity, of sperm into each morphometrical subpopulation in bull semen, therefore, the purpose of this study was to identify and characterize the morphometrically distinct subpopulations of cryopreserved bull sperm and to determine how sperm with damaged chromatin are distributed in each subpopulation, using a combination of toluidine blue stain
Patients were subject to Tissue Autofluorescence (Visually Enhanced Lesion Scope) and Toluidine Blue stain
01% toluidine blue stain
(Blue-McLendon and Green, 2010) and the results were expressed in units per microliter ([micro]L).
Sperm chromatin damage was assessed using toluidine blue stain
as described by Mello (1982) at
The log showed 11 (19-56%) positive margins taking up the toluidine blue stain
Leikola et al (10) examined 2 cohorts of patients with ILC, one using toluidine blue stain
and one using IHC.
The cells took up the toluidine blue stain
while the fibers remained translucent.
5% Toluidine Blue stain
can be quickly scanned by the technologist to determine if it is safe to stain the specimen with other cases.
The mass was composed of vascular spaces with scattered mast cells showing metachromatic reaction with toluidine blue stain
ii) Toluidine blue stain
(WHO Guidelines 2001b; Appendix 2-ix)
Samples were then processed routinely and stained with hematoxylin and eosin, in addition to acid mucicarmine and toluidine blue stains
to demonstrate mucin production.
Toluidine blue stains
mast cells red-purple (metachromatic staining) against a blue background.